中药引经成分β-细辛醚联合R8、MOG-ab修饰黄芪甲苷纳米粒的双级脑靶向递药系统的研究

多发性硬化症为中枢神经系统脱髓鞘疾病，黄芪甲苷(ASI)治疗该病有效，血脑屏障（BBB）透过率低。前期研究发现中药“引经”与穿膜肽可促进药物BBB的透过，但两者共为一级介导，与抗原介导的二级靶向结合进行双级脑靶向制剂的构建机制研究尚不清楚，课题组拟以脑靶向性好的中药引经成分β-细辛醚、穿膜肽R8为第一级靶向分子、髓鞘少突胶质细胞糖蛋白抗体MOG-ab为第二级靶向分子，构建一种经鼻给药、直接递送药物至髓鞘病变部位的双级脑靶向递药系统；采用水淬灭荧光染色法对递药系统的靶向性、靶向机制及各靶向基团的协同作用进行体内外评价，通过现代靶向递药技术与传统引经药性、鼻腔给药等多种策略联用，提高ASI脑内病变部位的递送效果，初步阐明穿膜肽与引经成分的一级介导协同作用、以及联合二级功能基的双级脑靶向的构建机制，为中药“引经”理论与现代靶向理论结合构建传统中药的双级脑靶向递药系统提供一种新的递药思路。

Multiple sclerosis is a demyelinating disease of the central nervous system．Astragaloside IV has better effect and lower Blood brain barrier permeability．Cell penetrating peptides and Chinese "Yinjing" can promote the drug through BBB．But the effect and mechanism of the combined construction of the first stage and the two stage targeting is not clear．The research group intends to build two-stage brain targeting drug delivery system that through intranasal administration．This system is composed of R8 and asarone for the first level target molecule， MOG-ab for the second stage．It can pass through the BBB and put the drug directly to lesions；We will evaluate on system’s targeting， targeting mechanism and the synergistic effect of targeted groups performed in vitro and in vivo by using the water quenched fluorescent dye．These studies can improve astragaloside brain lesion delivery effect，due to modern target to pass medicine technology and traditional resuscitation inducing aromatic herbs，nasal to the multiple target to methods combined．These can preliminary clarify the synergistic effect of receptor and its mediated components and the construction mechanism of Two stage brain targeting．These studies can also provid theory and experiment basis on the Chinese Medicine’s two-stage brain targeting drug delivery system，that combined by the “Yinjing” and targeting theories．