氧化还原酶在生物体的氧化还原反应中扮演着十分重要的角色。然而许多氧化还原酶的活化都需要辅酶NAD+/NADH的参与。采用光化学方法实现NAD+/NADH的再生可以将光催化和酶催化进行协同组合，是人类模拟和学习植物光合作用的重要方式。目前报道的NAD+/NADH再生大多面临着需要电子调节剂、以有机胺类为电子给体以及催化再生效率低等局限性。本项目拟以聚合物半导体为异相光催化剂，通过表面修饰以及化学改性，提高电子在光催化剂与NAD+之间的跃迁效率，实现NADH的高效再生。同时，开发可分散于水中的聚合物光催化剂，通过提高对水的氧化能力，用以解决以有机胺为电子给体的局面。在光催化活化NADH再生的基础上，引入生物酶实现光驱动酶催化有机化学反应。此外，通过限定氧化还原酶的空间位置，以光生电子为传递媒介，构建了光驱动多酶催化体系，实现化工产品和药物分子的精细化生产。

In nature, oxidoreductases play an essential role in redox reactions which happens in organisms. However, cofactors such as NAD+/NADH are frequently required for the activation of most oxidoreductases. Design of efficient NAD+/NADH regeneration system via photochemical approaches could synergistically combine the advantages of both photocatalysis and bioenzyme catalysis, which is an important way to simulate the natural photosynthesis in plants. However, there are some drawbacks for currently reported NAD+/NADH regeneration systems, such as electron mediator being needed, organic amines working as major electron donor and limited regeneration efficiency. This project intends to improve the NAD+/NADH regeneration efficiency via a photochemical approach by designing various polymer heterogeneous photoactive systems. The modification and functionalization of polymers could dramatically enhance the electron transfer between polymer photocatalyst and NAD+, which obviously favors to the NADH regeneration. In addition, various water-dispersible polymer photocatalysts with high oxidation potential are prepared in order to oxidize the water, where organic amine could be avoided for the regeneration of NADH. Based on the optimized conditions, different kinds of bioenzymes reaction systems could be thus constructed for organic transformations thought combining the regenerated NADH and oxidoreductases. Finally, several enzyme-catalytic systems are designed via confining the location of enzymes and employing electrons as mediator, which demonstrates its applications in the synthesis of fine chemicals and pharmaceuticals.