生活污水和工业废水的排放，导致受纳水体中广泛检出壬基酚、双酚A和三氯生等酚类内分泌干扰物，并在水生生物体内富集。酚类内分泌干扰物具有活性较强的酚羟基，使其在生物体内具有较高的转化和代谢效率，但前期研究多集中于生物富集和毒理效应研究，缺乏对转化产物和代谢途径的认识，因此难以全面评价其生态和人体健康风险。本项目以壬基酚、双酚A和三氯生为目标物，采用实验室恒流暴露系统，模拟稀有鮈鲫暴露于环境相关浓度目标物的情景，研究其鱼体代谢及毒代动力学过程。利用高分辨质谱鉴定其生物转化产物，明确母体及代谢物的鱼体组织分配及蓄积机制；测定相关代谢酶活性，并结合污染物在鱼肝微粒体的体外暴露实验，推断酚类内分泌干扰物在肝脏等组织的代谢途径；厘定关键生理生化参数，建立鱼体生理毒代动力学模型，预测鱼体中酚类内分泌干扰物及代谢物的蓄积水平，为此类物质的风险评价和化学品管控提供科学依据。

The discharge of domestic sewage and industrial wastewater leads to the wide detection of phenolic endocrine disrupting chemicals (EDCs), including nonylphenol, bisphenol A and triclosan, in receiving aquatic environment. Phenolic EDCs having a phenolic hydroxyl group with strong activity, which usually has high biotransformation ability and metabolism efficiency in organisms. Previous studies mostly focused on the bioaccumulation and ecological toxicity, while the knowledge on the metabolites and metabolism pathway of phenolic EDCs is lacking. Hence, it is difficult to fully assess their ecological and human health risks. Nonylphenol, bisphenol A and triclosan are selected as target compounds. The flow-through aquatic exposure systems are used to simulating the exposure of rare minnow (Gobiocypris rarus) to phenolic EDCs at environmentally relevant concentrations under laboratory conditions, and the metabolism and toxicokinetics in in fish tissues are evaluated. The biotransformation products of phenolic EDCs are identified using high resolution mass spectrometer. The fish tissue disposition and bioaccumulation mechanism of the parents and metabolites are evaluated. The activity of enzyme for the phases I and II metabolism are analyzed, and in vitro method of fish liver microsomes exposed to target compounds are carried out. The metabolic pathway of phenolic EDCs in liver and other tissues are proposed. Collecting and determining the key physiological and biochemical parameters, the fish physiology based toxicokinetic model are established, and the model will be used to predict the accumulation level of phenolic EDCs and the metabolites in fish. The results will contribute to providing the scientific basis for ecological and human health risk assessment, and hence supporting for the control of phenolic EDCs and chemicals with similar structures.