全球气候变暖是导致全球生物多样性下降的重要因素。在以往的研究基础上，本项目以分布在中国南北两个种群的变色树蜥为研究对象，结合野外研究和室内操纵实验，整合肠道微生物多样性分析、转录组学、生态免疫学和生活史特征，研究变色树蜥应对气候变暖，尤其是过冬阶段的能量分配策略。以检验以下假设：(1)与正常过冬的变色树蜥相比，遭遇暖冬的变色树蜥在冬眠期间会增加维持能耗，进而导致储存用于繁殖的能量下降，(2)冬眠后需要从外界获取能量，将影响产卵时间，(3)若产卵期后延，变色树蜥将倾向产较大而较少的卵，以增加后代适合度，(4)气候变暖将导致肠道菌群多样性下降，免疫能力也会出现相应改变，同时也将对个体能量代谢出现调控。本项目首次阐明蜥蜴应对全球气候变暖的生理生态反应，并望获得区别于其他类群的新的发现。

Global warming has been thought to be a key threat to biodiversity. Based on the findings of previous studies, we will investigate the responses of oriental garden lizards (Calotes versicolor) to global warming, especially the strategy of energy allocation during the winter, by analyzing gut microbiota, transcriptome, ecological immunology and life-history traits of individuals both in the field and in the laboratory. We hypothesize: (1) compared to individuals under the normal winter, individuals in warm winters will increase maintenance costs of energy and reduce energy allocation to reproduction, (2) individuals need to obtain energy from the environment after hibernation, thus altering the timing of egg-laying, (3) females that lay eggs later in the season will tend to produce larger but fewer eggs to enhance offspring fitness, (4) climate warming will change the diversity of gut microbiota, immune capacity and energy metabolism in C. versicolor. Our study is the first to explain the ecological and physiological responses to climate warming in lizards, and we hope to find something conceptually new that has never been reported for any other taxa.