Environment-Dependent Variation in Gut Microbiota of an Oviparous Lizard (Calotes versicolor).
Environment-Dependent Variation in Gut Microbiota of an Oviparous Lizard (Calotes versicolor).
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卵生蜥蜴（Calotes versicolor）肠道微生物群的环境依赖性变异
DOI：
10.3390/ani11082461
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发表时间：
2021-08-21
期刊：
影响因子：
--
通讯作者：
Dayananda B
中科院分区：
文献类型：
--
作者：
Zhang L;Yang F;Li N;Dayananda B
简单总结 不同的肠道部分可能为肠道微生物群提供不同的栖息地。我们发现拟杆菌门、厚壁菌门和变形菌门是云芝肠道微生物群的三个主要门。优势菌门拟杆菌门和厚壁菌门的相对丰度从小肠到大肠呈增加趋势，其中拟杆菌属（纲：拟杆菌属）、粪杆菌属和真杆菌属（纲：丹毒菌属）、副拟杆菌属（科：紫单胞菌科）和瘤胃球菌属（科：毛螺菌科）和科等丰度较高。后肠中存在Odoribacteraceae和Rikenellaceae，并且某些代谢途径在后肠中含量较高。我们的结果揭示了蜥蜴肠道不同部位肠道微生物群组成和代谢途径的变化。摘要 脊椎动物与其胃肠道内的微生物群保持着复杂的共生关系，这反映了宿主与其肠道微生物群之间的生态和进化关系。然而，这种理解仅限于蜥蜴，并且对宿主胃肠道内肠道微生物群的空间异质性和共现模式以及样本之间微生物群落的变化仍然知之甚少。为了解决这个问题并为蜥蜴肠道微生物群取样提供指导，我们研究了东方花园蜥蜴（Calotes versicolor）三个肠道位置的细菌，并通过 16S 核糖体 RNA (16S rRNA) 基因扩增子测序分析数据的细菌组成。我们发现优势门的相对丰度从小肠到大肠呈增加趋势，厚壁菌门、拟杆菌门和变形菌门是云芝肠道菌群的三个主要门。后肠样本中含有较高丰度的拟杆菌属（类：拟杆菌属）、粪杆菌属和真杆菌属（类：丹毒菌属）、副拟杆菌属（科：卟啉单胞菌科）和瘤胃球菌属（科：毛螺菌科）以及臭杆菌科和 Rikenellaceae 科。后肠的次级胆汁酸生物合成、糖胺聚糖降解、鞘脂代谢和溶酶体显着高于小肠。综上所述，我们的结果表明东方花园蜥蜴不同部位的肠道微生物群组成和代谢途径存在差异。
Simple Summary The different gut sections potentially provide different habitats for gut microbiota. We found that Bacteroidetes, Firmicutes, and Proteobacteria were the three primary phyla in gut microbiota of C. versicolor. The relative abundance of dominant phyla Bacteroidetes and Firmicutes exhibited an increasing trend from the small intestine to the large intestine, and there was a higher abundance of genus Bacteroides (Class: Bacteroidia), Coprobacillus and Eubacterium (Class: Erysipelotrichia), Parabacteroides (Family: Porphyromonadaceae) and Ruminococcus (Family: Lachnospiraceae), and Family Odoribacteraceae and Rikenellaceae in the hindgut, and some metabolic pathways were higher in the hindgut. Our results reveal the variations of gut microbiota composition and metabolic pathways in different parts of the lizards’ intestine. Abstract Vertebrates maintain complex symbiotic relationships with microbiota living within their gastrointestinal tracts which reflects the ecological and evolutionary relationship between hosts and their gut microbiota. However, this understanding is limited in lizards and the spatial heterogeneity and co-occurrence patterns of gut microbiota inside the gastrointestinal tracts of a host and variations of microbial community among samples remain poorly understood. To address this issue and provide a guide for gut microbiota sampling from lizards, we investigated the bacteria in three gut locations of the oriental garden lizard (Calotes versicolor) and the data were analyzed for bacterial composition by 16S ribosomal RNA (16S rRNA) gene amplicon sequencing. We found the relative abundance of the dominant phyla exhibited an increasing trend from the small intestine to the large intestine, and phyla Firmicutes, Bacteroidetes and Proteobacteria were the three primary phyla in the gut microbiota of C. versicolor. There were a higher abundance of genus Bacteroides (Class: Bacteroidia), Coprobacillus and Eubacterium (Class: Erysipelotrichia), Parabacteroides (Family: Porphyromonadaceae) and Ruminococcus (Family: Lachnospiraceae), and Family Odoribacteraceae and Rikenellaceae in the sample from the hindgut. The secondary bile acid biosynthesis, glycosaminoglycan degradation, sphingolipid metabolism and lysosome were significantly higher in the hindgut than that in the small intestine. Taken together our results indicate variations of gut microbiota composition and metabolic pathway in different parts of the oriental garden lizard.
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DOI：
10.1038/nrmicro3552
发表时间：
2016-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Donaldson GP;Lee SM;Mazmanian SK
通讯作者：
Mazmanian SK
影响因子：
15.5
作者：
Cao, Jian;Hu, Yongfei;Gao, George F.
通讯作者：
Gao, George F.
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
5.8
作者：
Kembel, Steven W.;Cowan, Peter D.;Webb, Campbell O.
通讯作者：
Webb, Campbell O.
微生物群-肠-脑相互作用调节雄性布氏田鼠（Lasiopodomysbrandtii）对寒冷的宿主代谢适应
影响因子：
11
作者：
Bo, Ting-Bei;Zhang, Xue-Ying;Wang, De-Hua
通讯作者：
Wang, De-Hua