Wolbachia 影响二斑叶螨生殖的细胞学及分子机理研究

二斑叶螨是农业生产上重要害虫，常造成严重危害。前期研究表明二斑叶螨普遍感染共生菌Wolbachia，Wolbachia能提高二斑叶螨的产卵量，引起胞质不亲和(CI)，然而其机理尚不清楚。本项目通过比较不同感染情况下二斑叶螨生殖细胞增殖及凋亡情况，明确Wolbachia影响二斑叶螨生殖力的细胞学机理；利用基因表达谱、miRNA表达谱、定量PCR等手段，并参考二斑叶螨基因组信息，发掘Wolbachia感染引起差异表达的二斑叶螨生殖发育相关功能基因，揭示Wolbachia影响二斑叶螨生殖力的分子途径，解析Wolbachia介导二斑叶螨基因表达产生CI的分子机理。研究结果将有助于阐明Wolbachia与寄主的互作机理，揭示二斑叶螨繁殖和暴发过程中Wolbachia发挥的作用，对有效控制二斑叶螨和其他害虫具有重要指导意义。

Tetranychus urticae is an important agricultural pest, which often causes serious damage to agricultural production. Previous studies have shown that T. urticae is widely infected by endosymbiont Wolbachia,which can enhance fecundity and induce cytoplasmic incompatibility (CI)in T. urticae. However,the mechanisms of Wolbachia's influence on reproduction in T.urticae remain unclear. In this study, the stem cell proliferation and apoptosis of infected females are compared with uninfected ones to elucidate the cytological mechanism of changed fecundity.By using gene expression profiles, miRNA expression profiles, quantitative PCR and other methods, and taking advantage of T. urticae genomic information, we'd like to get the differentially expressed functional genes of host associated with reproductive changes induced by Wolbachia, and clarify the molecular mechanism of fecundity changes and CI. The results will help us understand the interactions between Wolbachia and hosts, reveal the role of Wolbachia in the two-spotted spider mites's reproduction and outbreak, and guide the control of T. urticae and other pests.