马铃薯是世界第四大粮食作物，对维护我国粮食安全起着重要作用，然而由Phytophthora infestans所引起的晚疫病严重威胁着马铃薯生产。在过去几十年间，由R基因介导的马铃薯抗性往往由于RXLR类无毒基因的快速进化而迅速丧失。而PAMP在进化上非常保守，因此由PAMP诱导的抗性更为持久。INF1是一种研究地较为清楚的由P. infestans分泌的PAMP，近期在马铃薯野生种中成功分离克隆了INF1的受体基因ELR。前期研究证明，在感病马铃薯栽培种Désirée中超量表达ELR，室内接种条件下可以在一定程度上提高马铃薯对晚疫病的抗性。本项目旨在通过iTRAQ等蛋白质组学技术以及高通量基因功能分析筛选鉴定参与INF1和ELR互作免疫反应的调控基因。通过本研究进一步了解INF1和ELR互作免疫调控机制，为马铃薯晚疫病持久抗性育种提供理论基础和技术策略。

Potato is the fourth most important food crop in the world and plays an important role to the food safety of our country. But potato production severely suffers from late blight caused by Phytophthora infestans. In the past dozens of years, because of the fast evolution of RXLR effectors, R gene-mediated resistance can be quickly lost. In contrast, pathogen-associated molecular patterns (PAMPs) are much more conserved and thus can lead more durable resistance. INF1 is a well-characterized PAMP secreted by P. infestans. Its receptor called ELR (elicitin response) was recently successfully cloned from a wild potato. It has been proved that overexpression of ELR in susceptible potato cultivar Désirée can increase potato resistance to a certain level in a lab-controlled condition. The aim of this project is to screen and identify the regulatory genes involved in the interaction of INF1 and ELR by proteomics techniques such as isobaric tag for relative and absolute quantitation (iTRAQ) and high-throughput functional analysis. After this research, we can further understand the immunological mechanism based on the interaction of INF1 and ELR. This can provide fundamental basis and technology strategy for breeding durable resistant potato against late blight.