常规影像学检查在早期常难以鉴别肝癌射频消融治疗后的良性增强带与残余肿瘤。肿瘤血管与良性组织的血管不一样，其发育不成熟，对血管活性药物缺乏应答反应。去氧肾上腺素这种血管活性药物可引起皮肤、粘膜、内脏等处的血管收缩，继而导致血流灌注减少。有研究证实，去氧肾上腺素可使良性病灶的血流灌注减少，而不能显著改变恶性肿瘤的血流灌注。超声造影目前已被广泛用于肝癌射频消融术后的评估，而且其可以探索肝内正常组织和肿瘤的血流灌注。基于此，课题组先制成完全消融和不完全消融的兔VX2肝癌模型，再使用去氧肾上腺素负荷超声造影分别探索良性增强带与残余肿瘤在使用去氧肾上腺素前后的血流灌注变化，根据二者血流灌注变化的不一致去鉴别二者，然后与病理学检查的结果相对照。本课题旨在验证去氧肾上腺素负荷超声造影可能是鉴别肝癌射频消融术后良性增强带与残余肿瘤的一种良好方法，为肝癌射频消融术后的早期评估提供一种新策略。

Conventional imaging is often difficult to differentiate benign periablational enhancement (BPE) from residual tumor after radiofrequency ablation (RFA) for hepatocarcinoma. Tumor vessels which do not react to vasoactive drugs because they are immature, are different from the vessels of benign tissues. The vasoactive drug of phenylephrine could produce vasoconstriction of skin, mucous membrane and splanchnic, and accordingly lead to a marked decreasement of the blood perfusion. Previous studies have confirmed that phenylephrine could markedly decrease the blood perfusion of benign tissues, but had little effect on the malignant tumors. Contrast-enhanced ultrasonography (CEUS) is widely used to assess the effects of RFA on hepatocarcinoma, and could explore the blood perfusion of the tumor and liver tissue. Therefore, we established complete and partial RFA model in rabbit VX2 liver tumors, and utilized phenylephrine stress CEUS to explor the blood perfusion of BPE and residual tumor before and after phenylephrine administration respectively. Then we differentiated it according to the inconsistent blood perfusion change of BPE and residual tumor in response to phenyleprine. The results of phenylephrine stress CEUS examination were compared with the results of histopathologic examination. The aim of our programe is to verify that phenylephrine stress CEUS may be helpful in differentiating BPE from the residual tumor after RFA in hepatocellular carcinoma, and provide a new strategy for post-RFA assessment in hepatocellular carcinoma at an early stage.