保健灸对老年小鼠“长寿基因”SIRT1的调控

人口的老龄化是21世纪全球的社会难题。国务院制定了《中国老龄事业发展“十二五”规划》，布署了开展老年病的预防保健等重要任务。艾灸对老年病的预防保健（治未病），有确切的疗效，但其理论构建尚不完善。中医药管理局也制定了中医预防保健的纲要，以支撑和引领中医预防保健科学规范发展。.本课题以保健灸为切入点，引入国际老龄化和老龄化相关疾病的治疗靶点-“长寿基因”SIRT1，拟长期干预老年小鼠，严格监控艾烟浓度，观察小鼠的体重、血糖、血脂、学习记忆、活动等变化，并计算小鼠的患瘤率和生存期，评价艾灸的预防保健功效；采用先进的生物技术，对SIRT1蛋白和SIRT1及其数条调控通路中的数千条基因如FOXO3α、P53、NF-қB、PPAR、PGC-1α等，进行高效的筛选和规模化的识别，完善保健灸的防病保健的机制，为中医防病保健的社会认可提供科技支撑，为寻找中医防病保健的治疗靶点提供线索。

So far, the whole society faces a big problem of the growing population aging.the Sate Council issued the development of "China cause of aging of the Twelfth Five Year Plan"，to deploy the important tasks of the health care in elderly patients. moxibustion was effective as an antiaging therapy and modern research confirmed it.while, its theory is not perfect. Administration of traditional Chinese medicine has made traditional Chinese medicine prevention and health care program, to support and guide the Chinese preventive practice of Health Science development.This study focuses on moxibustion for health care. It would import the "longevity gene" SIRT1, which is the therapy target for aging and aging related diseases treatment. We quasi long term intervention in aged mice, with strictly control of moxa smoke concentration, to observe body weight, blood glucose, blood lipid, changes of learning and memory activities and tumor rate and survival to evaluat the health care efficacy. Using advanced biotechnology, thousands of genes and a number of regulatory pathways such as FOXO3α, P53,NF-қB, PPAR, PGC-1α, would be identified to improve the mechanism of moxibustion in health care, and to provide scientific and technological support for society recognition of health care with TCM, and provide new clues for therapeutic target for disease prevention..