原发性肝细胞癌是广西高发的恶性肿瘤。不同个体在同样的暴露环境，其肝癌发病率存在差异。本课题的前期研究提示，肝癌发生发展存在地域和种群差异与体内肿瘤微环境密切相关。肿瘤相关巨噬细胞（TAMs）是肿瘤微环境中的主要成员。本项目拟通过诱导培养出TAM1和TAM2，建立两者与肝癌细胞或正常肝细胞的三维立体共培养体系，运用iTRAQ与质谱相结合的定量蛋白质组学技术，分析TAM1和TAM2的外分泌蛋白组与TAMs和肝癌细胞相互诱导的蛋白质分子, 用临床标本对其中差异蛋白质分子进行表达验证，研究肝癌微环境中TAM1、TAM2外分泌蛋白对肝癌细胞生长和侵袭能力的影响，以期发现肝癌发生发展中新的分子标志物，为肝癌的早期诊断和靶向治疗提供理论基础和实验依据。

Primary Hepatocellular Carcinoma is a malignant tumor with high incidence in Guangxi province. The morbidity of hepatocellular carcinoma is different in individuals with the same exposure. Our previous studies indicated that this different incidence may dependent on the geographic regions and ethnic difference and tumor microenvironment may play a critical role in the development of HCC. Tumor associated macrophages (TAMs) are main component In tumor microenvironment. In this project, We plan to culture TAM1 and TAM2 in vitro by induction , build three-dimensional co-culture system of TAMs with hepatoma cells and normal liver cells, screen differentially expressed proteins by comparative analysis of protein molecules using iTRAQ and mass spectrometry combined with quantitative proteomics technology, then validate the differential proteins expression and analysis the functions of differentially expressed proteins in malignant tumor growth, metastasis, tumor immunity in cell signaling in this project, which will help us to explore the potential impact of proteins secretion changes of TAMs on hepatocarcinogenisis and invasiveness in hepatic microenvironment and search new molecular markers for liver cancer invasion and provide theoretical evidence for the treatment of metastatic HCC by TAMs targeting therapy.