Over-expressed Testis-specific Protein Y-encoded 1 as a novel biomarker for male hepatocellular carcinoma.
Over-expressed Testis-specific Protein Y-encoded 1 as a novel biomarker for male hepatocellular carcinoma.
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过度表达的睾丸特异性蛋白 Y 编码 1 作为男性肝细胞癌的新型生物标志物
DOI：
10.1371/journal.pone.0089219
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Li S;Mo C;Huang S;Yang S;Lu Y;Peng Q;Wang J;Deng Y;Qin X;Liu Y
肝细胞癌（HCC）是一种男性多发的癌症。先前的研究主要集中在肝癌的性别相关差异上，但其潜在机制仍不清楚。在这里，我们的目标是发现男性 HCC 的特征生物标志物。临床样本经过 iTRAQ 标记，然后进行 2DLC-ESI-MS/MS 分析。与女性HCC组相比，男性HCC组筛选出73个差异蛋白，其中16个上调蛋白和57个下调蛋白。睾丸特异性蛋白 Y 编码 1 (TSPY1) 在男性 HCC 中具有特征性特征，被选择用于进一步研究。 TSPY1 功能效应的数据表明，TSPY1 的过表达可以增强 HCC 细胞增殖，增加软琼脂定植，诱导更高的细胞侵袭能力，并与 HCC 细胞系的转移潜力相关。此外，TSPY1 和雄激素受体 (AR) 在 HCC 细胞系以及 HCC 组织中同时共表达。 TSPY1 上调或下调可能导致 AR 表达水平高或低。这些结果表明，TSPY1 可能参与男性 HCC 中 AR 表达的调节，并可能作为男性 HCC 的新型生物标志物。
Hepatocellular carcinoma (HCC) is a male-predominant cancer. Previous studies have focused on the sex-related disparity in HCC, but the underlying mechanism remains unclear. Here, we aimed to discover characteristic biomarkers for male HCC. Clinical samples were subjected to iTRAQ labeling followed by 2DLC-ESI-MS/MS analysis. Seventy-three differential proteins containing 16 up-regulated and 57 down-regulated proteins were screened out in the male HCC group compared to that in female HCC group. Testis-specific Protein Y-encoded 1(TSPY1) is characteristically present in male HCC and was chosen for further investigation. The data from the functional effects of TSPY1 indicated that over-expression of TSPY1 could potentiate HCC cell proliferation, increase soft agar colonization, induce higher cell invasive ability and correlate with the metastatic potential of the HCC cell lines. In addition, TSPY1 and androgen receptor (AR) were co-expressed simultaneously in HCC cell lines as well as in HCC tissue. TSPY1 up- or down-regulation could lead to a high or low level expression of AR. These results implied that TSPY1 may be included in the regulation of AR expression involved in male HCC and it may act as a novel biomarker for male HCC.
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影响因子：
8.8
作者：
Tian J;Tang ZY;Ye SL;Liu YK;Lin ZY;Chen J;Xue Q
通讯作者：
Xue Q
蛋白质组学表达的特征区分肝细胞癌中的癌性和非恶性组织。
影响因子：
4.4
作者：
Lee NP;Chen L;Lin MC;Tsang FH;Yeung C;Poon RT;Peng J;Leng X;Beretta L;Sun S;Day PJ;Luk JM
通讯作者：
Luk JM
影响因子：
4.4
作者：
Chong, Poh Kuan;Lee, Huiyin;Zhou, Jianbiao;Liu, Shaw-Cheng;Loh, Marie Chiew Shia;Wang, Ting Ting;Chan, Siew Pang;Smoot, Duane T.;Ashktorab, Hassan;So, Jimmy Bok Yan;Lim, Khong Hee;Yeoh, Khay Guan;Lim, Yoon Pin
通讯作者：
Lim, Yoon Pin
影响因子：
17.1
作者：
Wu MH;Ma WL;Hsu CL;Chen YL;Ou JH;Ryan CK;Hung YC;Yeh S;Chang C
通讯作者：
Chang C
影响因子：
6.4
作者：
Kido, Tatsuo;Lau, Yun-Fai Chris
通讯作者：
Lau, Yun-Fai Chris