腋臭是一种临床常见的常染色体显性遗传性疾病，因严重影响工作生活，一直是整形外科临床工作的热点。课题组前期研究发现转运蛋白MRP8的编码基因ABCC11及其多态性位点rs17822931不同等位基因型对腋臭的发生有更重要作用，但ABCC11基因编码转运蛋白MRP8不是腋臭形成的主要转运蛋白（ApoD），研究发现ABCC11与ApoD存在正相关性，因此推测ABCC11基因与ApoD可能存在影响，但具体作用机制仍不清楚。本研究运用分子生物学等方法进一步阐明ABCC11基因rs17822931不同等位基因型及其编码蛋白水平对顶泌汗腺增殖及分泌作用的影响，通过基因的过表达和沉默分析对载体蛋白ApoD的JNK1信号通路的影响，探讨腋臭的发病机制，为临床腋臭的诊断及治疗提供新方向及思路，具有重要意义.

Axillary osmidrosis (AO) is a common inherited disease characterized by its symptomatic unpleasant odor from axilla，which influence living quality. There are bottlenecks of bromhidrosis in clinical diagnosis and treatment that should be noticed: Firstly, the exact pathogenesis of bromhidrosis syndrome is still unclear. It is generally considered that axillary osmidrosis is related to the abnormal secretions of the apocrine sweat gland. Its secretion was carried to the skin surface and decomposited by bacteria, generating smelly unsaturated fatty acids. But the specific molecular mechanism is still unclear and the typical autosomal dominant hereditary is unexplainable. Therefore, further study is required to explore the genetic basis of bromhidrosis, which may delight new approaches for bromhidrosis treatment.Secondly, the current clinical diagnosis of bromhidrosis is primarily based on the patient's subjective feeling. Sometimes the presence of odor is difficult to determine especially for patients with less severe odor. And no uniform evaluation for treatments made the scientific comparison study with different methods invalid. Therefore, further study of the pathogenesis of bromhidrosis is still needed, which would helpful to find reliable indicators and related therapeutic drug. MRP8 is a vital protein encoded by ABCC11 gene,however,ApoD is the key transport protein during bromhidrosis.The co-relationship between ABCC11 and ApoD is studied, we suppose that ABCC11 influences ApoD through JNK1 signal pathwy.In our recearch,the apocrine sweat gland cells culture and vitro models establishment .The growth characteristics of different genotypes were observed and the proliferation was analyzed. The protein levels of ApoD and ABCC11/MRP8 of cell lines was analysed. The study will further study the mechanism of development of bromhidrosis.