盐敏感性高血压易感基因多态鉴定及其分子调控机制研究

高血压威胁全球人类健康，也是最重要的心血管疾病危险因素。除遗传作用之外, 盐始终被认为是高血压最重要的环境因素。人群内不同个体对盐负荷或限盐呈现不同的血压反应，即盐敏感性。鉴定盐敏感高血压易感基因有助于揭示原发性高血压发病机制。本申请前期工作开展人群盐敏感性高血压干预实验，完成了100个原发性高血压家系成员的盐敏感性测定和相关资料收集。拟采用高通量测序技术，在全基因组表达水平上筛选盐敏感高血压新的易感基因；并在大样本盐敏感干预个体中进行易感基因多态位点的关联研究，针对编码区或调控区的阳性多态进行生物学功能研究。旨在揭示盐敏高血压感易感基因的分子调控机制，有助于指导盐敏感高血压的个体化药物治疗和人群高血压的早期防治。

Hypertension is a threat to global human health, and is the most important cardiovascular disease risk factor.In addition to the genetic roles,salt has always been considered the most important environmental factor of hypertension. Different individuals show different blood pressure responses to salt loading or salt restriction, that is salt-sensitive. To identify the salt-sensitive hypertension susceptibility genes will contribute to reveal the pathogenesis of essential hypertension. Salt-sensitive in 100 essential hypertension family member has been determined, and related information clllected in salt-sensitive hypertension intervention experiment of preliminary work of this application. We intend to use high-throughput sequencing technology to screen new salt-sensitive hypertension susceptibility genes on a genome-wide expression levels, perform association study of susceptibility gene polymorphic loci in large samples of salt-sensitive individuals of intervention, and study the biological function of positive polymorphism of the coding region or regulatory region. This application is designed to reveal the molecular mechanism of salt sensitive hypertension susceptibility genes, contributing to individualized drug treatment of salt-sensitive hypertension and early prevention and treatment of hypertension of population.