家畜的繁殖潜能主要取决于卵泡发育，而卵泡发育的命运（成熟或闭锁）主要是由卵泡内颗粒细胞的状态（增殖或凋亡）决定的。根据前期研究结果和文献分析发现miR-1275、LRH-1和CYP19A1三者之间存在内在联系，且与猪卵泡闭锁、E2含量有密切相关，因此我们推测三者可以形成一个新的信号通路：miR-1275/LRH-1/CYP19A1通路，而且可能在猪卵巢颗粒细胞凋亡和雌激素合成中发挥作用。基于以上假设，本项目拟采用过表达等方法进一步明确猪卵巢颗粒细胞中miR-1275/LRH-1/CYP19A1通路中信号分子间的调控关系，采用ChIP等技术分析通路信号分子间的调控机制，采用FACS等技术研究通路在猪卵巢颗粒细胞凋亡和雌激素合成中的作用。研究对从表观遗传学角度阐明卵泡发育、揭示猪繁殖力性状形成的分子机理和提高猪繁殖力等均有一定意义。

The reproductive performance of livestock depends on the development of follicles, while the fate of the follicles decided by the state of granulosa cells (proliferation or apoptosis). Previous studies showed that there are internal relations among miR-1275, LHR-1 and CYP19A1, moreover, they have a close association with porcine follicular atresia and the content of E2. Therefore, we speculate that they could form a new signaling pathway: miR-1275/LHR-1/CYP19A1 pathway, which may play an important role in the porcine granulosa cell apoptosis and the estrogen synthesis. Base on the assumption, this project intends further to clarify the regulation relationship among miR-1275/LHR-1/CYP19A1 signaling molecules by multiple methods such as overexpression and ChIP. FACS and other methods will be used to analysis the effect of the pathway in porcine granulosa cell apoptosis and estrogen synthesis. The study would significantly elucidate the follicular development process, reveal the molecular mechanism for porcine reproduction and contribute to porcine fertility improvement.