大麦蛋白质含量主效QTLQGpc.ZgAx-6H.1的精细定位及候选基因克隆和功能鉴定

蛋白质含量是大麦重要的品质指标之一。我们利用蛋白质含量有显著差异的大麦品种紫光芒裸二棱（高蛋白质含量）和澳选3号（低蛋白质含量）构建的重组自交系群体，在6HL染色体上检测到1个控制蛋白质含量的主效QTL（QGpc.ZgAx-6H.1）。本研究拟在此基础上通过已经构建的近等基因系群体对主效QTL进行精细定位，克隆大麦蛋白质含量候选基因并进行转基因功能验证，利用近等基因系进行候选基因不同组织器官的表达模式分析，初步明确蛋白质含量基因的功能。另外，还将对蛋白质含量QTL/基因的育种利用价值进行系统评价，以期为大麦籽粒蛋白质含量的遗传改良奠定基础。

Grain protein content is one of the important quality traits in barley. Two major QTLs (QGpc.ZgAx-6H.1) controlling grain protein content were detected on barley chromosome 6HL, which have been identified by using a population of recombinant inbred lines derived from Ziguangmangluoerleng and Aoxuan 3 with contrasting grain protein content in our previous study. In the project, two major QTL will be fine mapped and candidate genes will be positional cloned by using a population of near-isogenic lines (NILs). Furthermore, the function of candidate genes will be characterized by transgenic analysis strategy and NILs lines of candidate gene will be selected for expression pattern, with the purpose to elucidate the underlying the function of grain protein content. In addition, the breeding value of these QTL/gene will be also analyzed in detail, and laying a foundation for the genetic improvement of barley grain protein content.