Regulation of nitrogen availability results in changes in grain protein content and grain storage subproteomes in barley (Hordeum vulgare L.)
Regulation of nitrogen availability results in changes in grain protein content and grain storage subproteomes in barley (Hordeum vulgare L.)
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氮有效性的调节导致大麦（Hordeum vulgare L.）谷物蛋白质含量和谷物储存亚蛋白质组的变化
DOI：
10.1371/journal.pone.0223831
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发表时间：
2019-10
期刊：
影响因子：
3.7
通讯作者：
Rugen Xu
中科院分区：
文献类型：
--
作者：
Baojian Guo;Dongfang Li;Sen Lin;Ying Li;Shuang Wang;Chao Lv;Rugen Xu
大麦籽粒蛋白质含量是决定籽粒最终利用价值的重要品质因子。籽粒蛋白质的合成和积累高度依赖于氮肥的有效性，了解其潜在的控制机制具有重要意义。本研究对洋思麦3号和纳索二条大麦成熟籽粒种子的GPC和蛋白质组成进行了分析。比较了低氮和高氮条件下籽粒贮藏亚蛋白质组（白蛋白、谷蛋白、蛋白和谷蛋白）的差异。施氮处理后，杨思麦3号的成熟粒GPC显著高于二条稻。白蛋白、玉米糖蛋白和谷蛋白含量在羊思麦中均有增加，而在二条菜中只有玉米糖蛋白含量增加。对粮食储存亚蛋白质组的大规模分析发现，在pH范围为3-10的2-DE凝胶上有152个差异表达的蛋白点。其中，在低氮和高氮条件下，杨思麦3号和二条菜成功鉴定了42个和66个蛋白点。所鉴定的蛋白质根据其生物学功能进一步分为13类。对籽粒亚蛋白质组的详细分析提供了大麦GPC如何受氮供应控制的信息。
Barley grain protein content (GPC) is an important quality factor that determines grain end-use value. The synthesis and accumulation of grain protein is highly dependent on the availability of nitrogen fertilizer, and it is important to understand the underlying control mechanisms of this. In the current study, the GPC and protein composition of mature grain seeds from Yangsimai 3 and Naso Nijo barley cultivars were analyzed. Grain storage subproteomes (albumin, glubulin, hordein and glutelin) were compared in the cultivars grown in both low and high nitrogen level conditions. The GPC of mature grain was significantly higher in Yangsimai 3 than Naso Nijo following nitrogen treatment. Albumin, hordein and glutelin content were increased in Yangsimai, while only hordein content was increased in Naso Nijo. Large-scale analysis of the grain storage subproteome revealed 152 differentially expressed protein spots on 2-DE gels with a pH range of 3–10. Among these, 42 and 66 protein spots were successfully identified by tandem mass spectrometry in Yangsimai 3 and Naso Nijo grown in low and high nitrogen conditions. The identified proteins were further grouped into thirteen categories according to their biological functions. This detailed analysis of grain subproteomes provides information on how barley GPC may be controlled by nitrogen supply.
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影响因子：
2
作者：
Huang TL;Sung ML;Chen TY
通讯作者：
Chen TY
影响因子：
2
作者：
Vensel WH;Tanaka CK;Altenbach SB
通讯作者：
Altenbach SB
通过 DIGE 对具有不同过滤性的大麦 (Hardeum vulgare) 麦芽差异进行蛋白质组学分析
影响因子：
3.3
作者：
Jin, Zhao;Li, Xiao-Min;Lu, Jian
通讯作者：
Lu, Jian
影响因子：
3.7
作者：
Sun H;Cao F;Wang N;Zhang M;Mosaddek Ahmed I;Zhang G;Wu F
通讯作者：
Wu F
影响因子：
7.4
作者：
Finnie, C;Melchior, S;Svensson, B
通讯作者：
Svensson, B