NF-kB信号通路在磨损颗粒诱导炎症性骨溶解中的作用研究

无菌性松动是人工关节关节置换术的重要并发症,这种无菌性松动严重阻碍了人工关节的推广应用，如何有效地通过非手术方式来预防和治疗人工关节无菌性松动已成为关节外科领域的研究热点。无菌性松动发生的生物学基础是磨损颗粒诱发的一系列炎症反应及破骨细胞的活化导致的骨溶解。前期研究表明，核转录因子kappaB （NF-kB）信号通路在炎症发生及破骨细胞的活化中都起到了重要作用。本研究将利用无菌性骨溶解的动物模型，探讨NF-kB信号通路在磨损颗粒诱导的炎症性骨溶解中的作用，并利用干扰技术对NF-kB p65亚基进行抑制，从而观察其对炎症反应及骨溶解的缓解作用。本研究将为人工关节置换术后无菌性松动的生物学治疗领域提供理论基础和新的思路。

Arthroplasty is the most effective means of treatment of severe joint disease, aseptic loosening is an important complication of arthroplasty, aseptic loosening a serious impediment to the promotion and application of artificial joints. How effective non-surgical ways to prevent and treat aseptic loosening has become the hot spot of research in the field of joint surgery. The biological basis of aseptic loosening is osteolysis caused by wear particlesinduced inflammation and osteoclast cell activation. Preliminary studies show that the nuclear transcription factor{kappa} B (NF-kB) signaling pathway play an important role in inflammation and activation of osteoclasts. In this study, the use of aseptic osteolysis in animal models to explore the role of NF-kB signaling pathway in wearparticle-induced inflammatory osteolysis, and interference technology to inhibition of NF-kB p65 subunit, in order to observe its effect on inflammation and osteolysis mitigation. The field of biological therapy for the aseptic loosening of artificial joint replacement surgery in this study provide a theoretical basis and new ideas.