宫颈癌是最常见的妇科恶性肿瘤之一，放疗是晚期宫颈癌的主要治疗手段，也是具有高危因素早期患者术后主要的辅助治疗手段，然而结果并不理想，主要原因是宫颈癌细胞出现放射抵抗。本课题组前期研究发现，纳米金球具有放疗增敏作用，与葡萄糖耦联后，能靶向性增加肿瘤细胞对纳米金颗粒的摄取。肿瘤相关钙离子信号转导因子2(TROP2)是近年来发现高表达上皮源性癌症细胞表面的糖蛋白，初步研究发现，TROP2在正常宫颈组织中表达量很低，而在宫颈癌中高表达。本研究拟合成新型纳米金材料-纳米金笼，并在其表面修饰TROP2单克隆抗体，形成靶向纳米金颗粒，利用肿瘤细胞表面抗原抗体复合物介导的内吞作用实现纳米金的主动靶向和被动靶向，探讨TROP2靶向性纳米金对宫颈癌的放疗增敏作用，光热治疗联合放疗的协同作用，进一步研究纳米金放疗增敏所致细胞凋亡相关分子机制，为以纳米技术结合特异单抗增强宫颈癌放疗增敏奠定基础。

Cervical cancer is one of the most common gyecologic cancers among women worldwide. Radiotherapy plays a major role in the treatment of patients with high risk and advanced cervical cancer，but the outcome is unsatisfactory, due to the cancer cells turned to be radio-resistant. Our preliminary study found that the gold nanoparticles have radiosensitization effect, when conjugated with glucose they could target to the tumor cell surface. Recent studies have reported that aberrant expression of TROP2 in a variety of human epithelial cancers is associated with tumor aggressiveness and poor prognosis. Our recent study found that TROP2 was highly expressed in cervical cancer, and decreased expression was found in normal cervical tissue. In this study, we will synthesis a novel metallic nanomaterial - Au nanocages, and conjugate it with TROP2 monoclonal antibody to form TROP2 targeted nanocages. The aim of our research is to study if the antigen-antibody complex mediated endocytosis could promote Au nanocages enter tumor cells by both active and passive targeting, photo-thermal therapy combined with radiotherapy will improve the effect of radiosensitivity of Au nanocages, and further research the molecular mechanisms of Au nanocages induced enhancement of radiosensitivity.