上转换-二氧化钛红外光催化降解呕吐毒素作用机理及产物安全性评价研究

呕吐毒素是小麦赤霉病病原菌禾谷镰刀菌产生的一种真菌毒素，会对粮食作物造成严重污染，并极大的危害人体健康。对呕吐毒素的阻控、降解成为食品质量安全控制研究的热点。光催化降解技术是一种高效、安全的氧化技术，将其应用于呕吐毒素的降解具有显著优势。但现有的二氧化钛光催化剂受到自身禁带宽度限制，仅能在紫外光辐照下进行催化反应，太阳能中红外光未得到有效利用；并且光催化降解呕吐毒素作用机制及产物的安全性尚不明确。本项目拟合成上转换-二氧化钛复合光催化剂，以呕吐毒素为降解模型，研究在红外光辐照下的光催化降解行为；分析鉴定呕吐毒素光催化后的各级降解产物，推导光催化反应历程，阐明降解作用机理；建立细胞模型和动物模型，通过细胞毒理实验、免疫组化法及生理病理学分析等技术，阐明呕吐毒素降解产物对肠道细胞的毒性作用和对肠道组织的损伤情况，评价产物安全性。本研究将为光催化技术在真菌毒素降解中的应用提供理论依据和指导。

Deoxynivalenol (DON) is a mycotoxin mainly produced by the fungi Fusarium graminearum. Many food crops are contaminated by DON, which leads to considerable damage to human health. Therefore, it has been an advanced research hotspot to control and degrade DON in food safety and quality control. Photocatalytic degradation is an oxidation technology with high efficiency and safety, and it has significant advantages in the application of DON degradation. TiO2 photocatalyst induced photocatalytic degradation only under UV radiation exposure, and the infrared light has not been used efficiently due to the limitation of band-gaps. Moreover, whether the products of photocatalytic degradation are safe to human and environment are yet unclear. Therefore, upconversion-TiO2 photocatalyst would be synthesized and its photocatalytic degradation behaviors would be studied under infrared light radiation exposure using DON as a model. All levels of degradation products of DON would be analyzed and identified. The process and mechanism of photocatalytic degradation of DON would be studied according to the structure of products. In addition, the cell models and animal models would be established. Whether the products of DON degradation have a harmful effect on intestinal cells and intestinal tissue would be illuminated by using technologies of cytotoxicology, immunohistochemistry, and physiology and pathology. Thus, the safety of the products of photocatalytic degradation can be evaluated. The research would provide theoretical basis and guidance for the application of photocatalysis technique for mycotoxins degradation.