揭示水稻抗白叶枯病的分子机理，培育抗性水稻品种，有利于保障我国粮食安全。研究表明植物SnRK2基因家族参与ABA信号调控并响应生物胁迫与非生物胁迫，但是水稻中SnRK2家族成员OsSAPK的抗病分子机理尚不明确。前期研究中，申请人通过对RNAi和过表达植株的表型鉴定发现OsSAPK9正向调控水稻对白叶枯病的小种专化抗性，利用酵母双杂交文库筛选获得抗病相关蛋白OsSGT1，并通过蛋白质互作验证及表达分析初步证明OsSAPK9与OsSGT1互作调控水稻白叶枯病抗性反应。本研究拟运用LC-MS/MS、转录组测序、X-射线衍射等技术探明OsSAPK9介导的水稻抗病反应是否依赖于ABA，明确OsSAPK9与OsSGT1互作的分子机制并解析二者蛋白复合体的晶体结构，揭示OsSAPK9调控水稻抗病性的分子调控网络。本研究的实施有助于揭示水稻抗病性的分子机理，为培育抗病水稻品种提供新的基因资源和理论依据。

Revealing the pathogenic mechanism of bacteria blight is essential to improvement of resistant varieties, which ensures the food security in China. The sucrose non-fermenting1-related protein kinase 2 (SnRK2s) gene family members are involved in the regulation of abscisic acid (ABA) and response to both biotic and abiotic stresses in plant. However, in rice, the molecular mechanisms are still not clear. In our previous study, we found that OsSAPK9, a SnRK2 member, played a positive role in improving race-specific resistance for bacterial blight in rice. Moreover, OsSAPK9 could interact with OsSGT1 (Suppressor of G2 allele of skp1) both in vivo and in vitro, suggesting that OsSAPK9 and OsSGT1 might form a protein complex to regulate the defense response. In this study, we aim to ascertain whether ABA contributes to the OsSAPK9-mediated defense response, understand the possible molecular mechanisms of bacteria blight resistance mediated by the protein complex of OsSAPK9 and OsSGT1, uncover the putative gene regulatory networks underlying OsSAPK9-mediated defense response, present the crystal structure of the OsSAPK9-OsSGT1 protein complex by LC-MS/MS, transcriptomics, and X-ray diffraction method, etc. Our study will enrich the gene resources of disease-resistance, and provide new information and strategy for improvement of disease resistance in rice.