肌肉损伤是超强度或不适当运动、劳损等原因造成的一类常见的软组织损伤，其发病率仅次于感冒。按摩对肌肉损伤修复有独到的治疗作用，得到中外同仁一致认同，其疗效确切。目前研究发现推拿可促进相应组织肌力的改善及血流变学、生物力学等的改良变化。但在康复疗效的基础理论研究方面，有关按摩对组织细胞形态修复及功能恢复的机制尚待阐明。本研究用动物模型模拟意外碰撞条件下造成的肌肉损伤，通过对损伤的股四头肌进行按摩刺激，结合应用高频超声、微泡超声影像识别技术、蛋白质免疫组化技术、基因芯片技术比较研究按摩刺激与自然恢复情况下，对损伤组织微循环状况及损伤组织本身修复的影响，从形态学角度试图探究按摩刺激信号对急性肌肉损伤局部血液微循环体系重建的初步作用；再通过免疫组化技术、基因芯片技术、肌电技术等手段，研究与肌肉损伤相关的酶学变化、基因表达、肌电水平等改变，从功能变化的角度探究按摩对损伤肌肉细胞功能修复的作用机制。

Acute muscular injury is a kind of common soft tissue injury that is caused by superstrength movement、unfit movement and strain.Its incidence ranks only second to influenza. Massage has unique effect on muscular injury.Until now,it is reported that massage can improve muscular power、hemorheology and biomechanics.However,the underlying mechanism of massage on repairing cell morphology and function recovery remains unknown..The procotol is designed as follows: to establish a model of muscular injury by accidental colllison and treat the damaged quadriceps femoris by massage;compare the effect of massage treatment and spontaneous recovery on muscular injury microcirculation by high frequency、microbubbles ultrasonography identifcaiton technology、immunohistochemistry and gene chip technology; detect the effect of massage therapy on muscular tissue microcirculation reconstruction by morphology technology;research the enzymatic change related to muscular injury,gene expression and myoelectricity by immunohistochemistry、gene chip and myoelectric technology；analyze the mechanism of massage therapy on damaged muscular cell function recovery.