本课题组前期研究发现锰超氧化物歧化酶(SOD2)和干细胞标志物(Bmi1)的异常表达均与舌鳞癌的发生发展、转移及预后密切相关，SOD2与Bmi1的表达存在正相关关系，SOD2可通过H2O2-Snail信号通路调控舌鳞癌的侵袭转移。为了进一步研究SOD2/Bmi1在舌鳞癌干细胞侵袭转移中的作用及其机理，本课题将开展：①研究SOD2/Bmi1调控舌鳞癌侵袭转移是否与干细胞相关；②研究SOD2/Bmi1在舌鳞癌干细胞侵袭转移中的作用；③研究C-myc靶向调控SOD2和Bmi1及其调控舌鳞癌干细胞侵袭转移的能力。本研究阐明了C-myc-SOD2/Bmi1信号通路在舌鳞癌干细胞侵袭转移中起重要作用，C-myc/SOD2/Bmi1有望成为舌鳞癌侵袭转移的分子标志物，在舌鳞癌的诊断、预后预测以及分子治疗靶点中具有重要的应用价值，这对提高舌鳞癌患者的生存率具有重要的意义。

In our previous studies we had found that the deregulation of manganese superoxide dismutase (SOD2) and stem cell marker Bmi1(B-cell-specific Moloney murine leukemia virus insertion site 1) were related to the development、metastasis and prognosis of tongue squamous cell carcinoma (TSCC). There existed correlationship between the expression of SOD2 and Bmi1. SOD2 mediated the migration and invasion of TSCC via H2O2-dependent Snail signaling (Free Radic Biol Med 2012,53:44. BMC Cancer 2010, 10:365 Cancer Genomics Proteomics 2008,5:85. Cancer Genomics Proteomics 2009,6:131.)。In order to further evaluate the role of cancer stem cells and C-myc-SOD2/Bmi1 pathway in the metastasis of TSCC, at first we investigate whether the migration and invasion of TSCC mediated by Bmi1 and SOD2 is related to cancer stem-like cell (CSC); then we investigate the role of SOD2 and Bmi1 in the invasion and migration of stem cells in TSCC; finally we investigate whether C-myc regulates SOD2 and Bmi1 and mediates the metastasis of TSCC. In our study we will clarify that tongue cancer stem cells play an important role in the migration and invasion of TSCC. C-myc directly regulates Bmi1 and SOD2, and then mediates the migration and invasion of CSC in TSCC. Thus, our study will provide reliable biomarker for the diagnosis, prognosis prediction and target treatment in oral squamous cell carcinoma. In furture, this is very important to improve the survival of patients with tongue cancer.