氧化应激胁迫下大薯花青素调控五指山断奶仔猪肠道菌群分布及代谢机制

大薯（Dioscorea alata L.），又名参薯，是薯蓣科薯蓣属植物，种植于热带、亚热带地区，紫大薯块茎中含有一定量的花青素（Anthocyanidin ，CAN）（本课题组前期研究得出紫大薯鲜样块茎中花青素含量约3.32mg/g），其属于多酚类物质，广泛存在于蓝莓、葡萄、树莓等植物中。大量在小鼠上的研究表明花青素通过平衡氧化与抗氧化稳态发挥抗炎，预防癌症和肥胖等营养代谢病。花青素在猪生产尤其是断奶仔猪预防治疗肠炎的研究越来越受到业内关注和重视。但是由于结构、聚合度以及饲料组分和其他类黄酮物质的影响或动物或人的个体差异，自然界中存在的花青素结构多样，受到植物基因型的影响，而其生理功能又受到结构影响从而导致不同来源花青素研究结果不尽相同，海南地区种植的紫色大薯中含有的花青素结构及功能鲜有报道。在养猪生产中断奶仔猪氧化应激尤为常见，并影响动物生产效益，尤其海南地处热带亚热带地区，气候炎热，氧化应激问题更为突出。针对以上问题本项目选用海南产紫大薯为原料，采用醇提法和大孔树脂法提取并纯化花青素，以五指山猪断奶仔猪为实验动物，结合宏基因组学和代谢组学研究紫大薯花青素调控LPS诱导氧化应激胁迫下肠道微生物变化和代谢，并鉴定出差异功能菌和关键基因，在此基础上利用无菌五指山断奶仔猪对比分析，分析宿主临床表现和微生物代谢关系。

Dioscorea alata L., called also yam, belong to plant of Dioscorea L. in Dioscoreaceae, is planted tropical and subtropical areas. Purple tubers contain anthocyanins (CAN), which belong to poly-phenols , widely found in blueberries, grapes, raspberries and other plants. A lot of research in mice have shown that anthocyanins exerted anti-inflammatory, prevention of cancer and obesity and other nutritional metabolic disease by balancing oxidation and anti - oxidation steady state. Studies anthocyanins about enteritis were paid attention in weaned piglets. However, the results of anthocyanins from different sources were inconsistent due to the structure, degree of polymerization, feed components and other flavonoids or individual animals or people . acidified ethanol was used to extract anthocyanins in Purple tubers of Dioscorea alata L. from Hainan and purified by AB-8 macroporous resin ; anthocyanins regulate gut microbial communities and metabolism of LPS induced WUZHISHAN-weaned-piglets oxidative stress with metagenomic and metabolom technique, then the functional bacteria and key genes were identified , at the same time sterile WUZHISHAN-weaned-piglets were used to compare and analyze in order to build the relationship between host clinical manifestations and microbial metabolism, reveal the physiological metabolic mechanism of anthocyanin mediated intestinal microorganism regulation of oxidative stress.