Pre-Protection and Mechanism of Crude Extracts from Dioscorea alata L. on H(2)O(2)-Induced IPEC-J2 Cells Oxidative Damage.
Pre-Protection and Mechanism of Crude Extracts from Dioscorea alata L. on H(2)O(2)-Induced IPEC-J2 Cells Oxidative Damage.
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DOI：
10.3390/ani13081401
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发表时间：
2023-04-19
期刊：
影响因子：
3
通讯作者：
Wang, Xuemei
中科院分区：
文献类型：
--
作者：
Yun, Yanhong;Shi, Huiyu;Wang, Yanyu;Yang, Fengyuan;Zhang, Yuanxin;Feng, Haibo;Chen, Junpu;Wang, Xuemei
本研究采用的植物名称为“花叶阿拉塔L.”已知含有丰富的原花色素和花青素，其表现出能够清除自由基的有效抗氧化性质。此外，这些化合物已显示在药物毒性范围内有效促进IPEC-J2细胞的增殖。相反，暴露于H2 O2会损害IPEC-J2细胞，并引发细胞抗氧化酶活性增加。本研究考察了不同浓度的白藜芦醇粗提物对阿拉塔L.对IPEC-J2细胞抗氧化能力的影响，探讨了阿拉塔L.通过检测其对NF-κB炎症信号通路的影响，对抗H2 O2诱导的氧化损伤。研究了阿拉塔L.研究表明，花青苷等多种具有生物活性的化学成分，因此，研究花青苷对阿拉塔L.以及其粗提物在氧化应激之前对细胞的作用。为了建立合适的氧化损伤模型，使用H2 O2作为氧化剂建立IPEC-J2细胞损伤模型。当H2 O2浓度为120 μmol/L，损伤时间为8 h时，细胞存活率下降至70%左右，细胞出现明显的氧化应激反应。此外，还对阿拉塔L.通过提高总抗氧化能力（T-AOC）和过氧化氢酶（CAT）活性，增加总超氧化物歧化酶（T-SOD）及其基因的表达，降低丙二醛（MDA）含量和谷胱甘肽过氧化物酶（GSH-PX）活性及其基因的表达，促进葡萄糖转运蛋白SGLT 1基因的表达，同时降低GULT 2基因的表达，从而促进花青素进入细胞。此外，50 μg/mL的粗提物有效地抑制IκB和p65蛋白的磷酸化，从而降低细胞氧化应激。鉴于这些研究结果，藜阿拉塔L.可作为一种天然抗氧化剂用于实际育种和生产目的，本试验中粗提物的最佳浓度为50 μg/mL。
The Dioscorea alata L. is known to contain abundant proanthocyanidins and anthocyanins, which exhibit potent antioxidant properties capable of scavenging free radicals. Additionally, these compounds have been shown to effectively promote the proliferation of IPEC-J2 cells within a pharmacotoxicity range. Conversely, exposure to H2O2 can damage IPEC-J2 cells and trigger increased activity of cellular antioxidant enzymes. This study investigated the impact of crude extract from Dioscorea alata L. on the antioxidant capacity of IPEC-J2 cells and explored the pre-protective mechanism of Dioscorea alata L. against oxidative damage induced by H2O2 through an examination of its effect on the NF-κB inflammatory signaling pathway. The purple tubers of Dioscorea alata L. have been found to contain a variety of bioactive chemical components, including anthocyanins, which make it significant to investigate the pre-protective effects of Dioscorea alata L. and its crude extracts on cells prior to oxidative stress. To establish a suitable oxidative damage model, an injured model of IPEC-J2 cells was created using H2O2 as the oxidant. Specifically, when the concentration of H2O2 was 120 μmol/L and the injured time was 8 h, the survival rate of cells decreased to approximately 70%, and the cells exhibited a noticeable oxidative stress reaction. Moreover, the crude extracts of Dioscorea alata L. demonstrated beneficial pre-protective effects on IPEC-J2 cells by increasing the total antioxidant capacity (T-AOC) and catalase (CAT) activities, augmenting the expression of total superoxide dismutase (T-SOD) and its genes, reducing the content of malondialdehyde (MDA) and the activity of glutathione peroxidase (GSH-PX) and its expression of genes, and promoting the expression of glucose transporter SGLT1 gene while reducing that of GULT2 gene, thereby facilitating the entry of anthocyanins into cells. In addition, the 50 μg/mL crude extracts effectively inhibited the phosphorylation of IκB and the p65 protein, thus reducing cellular oxidative stress. Given these findings, Dioscorea alata L. can be considered a natural antioxidant for practical breeding and production purposes, with an optimal concentration of crude extracts in this experiment being 50 μg/mL.
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影响因子：
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作者：
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影响因子：
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作者：
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