近年来，与心血管密切相关的内皮功能障碍及表观遗传学研究成为当前热点。其中，cZNF609/mir-615-5p/靶基因信号通路在血管内皮细胞生长、迁移和维持血管形态稳定中发挥重要作用，并参与动脉粥样硬化病理进程。本课题组前期研究发现，缺血性脑卒中人群外周血单核细胞中cZNF609表达水平显著低于高血压人群，而在脑卒中预后又恢复上调。此外，相对脑卒中首发人群，其预后阶段靶基因Tie2和IGF2水平下调，MEF2A水平上调。表明该信号通路可能参与高血压和脑卒中发病及预后过程。本课题拟在前期基础上，进一步增加健康对照，并扩大样本，检测mir-615-5p和靶基因mRNA及蛋白水平，同时分析靶基因遗传变异与血压变化及高血压和脑卒中的关系，探讨cZNF609/mir-615-5p/靶基因信号通路在高血压和缺血性脑卒中发病中的分子机制研究，为高血压及脑卒中临床疗效及预后评估提供新的分子标志物。

Recently, the research of endothelial dysfunction and epigenetics, which is closely related to the cardiovascular diseases, has become a hot spot. The cZNF609/mir-615-5p/target genes signaling pathway plays an important role in the proliferation and migration of vascular endothelial cells as well as participates in the pathological process of atherosclerosis. Our previous study found that in peripheral blood monocytes cZNF609 expression of ischemic stroke (IS) patients was significantly lower than that of the matched hypertension cases, whereas increased significantly in 5-7 days of stroke onset. The gene expression levels of Tie2 and IGF2 were down-regulated in IS patients, while the gene expression level of MEF2A was up-regulated in 5-7 days of stroke onset. The results showed that the signal pathway may be involved in hypertension and stroke and its process of prognosis. Based on previous experiments, we will explore the molecular mechanism of cZNF609/mir-615-5p/target gene signaling pathway in the pathogenesis of hypertension and stroke by detecting the gene expression and protein levels of mir-615-5p and target genes in a larger sample size. It provides new molecular markers for the clinical efficacy and prognosis of hypertension and stroke.