基于体外消化/Caco-2细胞模型的有色金属区及附近区域蔬菜中砷对人体的生物有效性研究

我国因有色金属开发导致蔬菜砷污染对人体健康造成威胁，广西有色金属矿区周边土壤砷污染引起的蔬菜砷污染问题尤为突出。砷生物有效性是科学评价砷对生物毒性和人体健康风险的重要指标。然而，目前评价砷生物有效性的体系并不成熟，对我国有色金属矿区及其周边区域蔬菜砷的生物有效性研究较少。因此，本项目拟开展以下几方面的研究：（1）蔬菜砷生物有效性的体外消化/Caco-2细胞模型方法体系的构建；（2）有色金属矿区及附近区域蔬菜砷生物有效性及其对人体健康风险；（3）中式烹饪对有色金属区周边蔬菜中砷的生物有效性的影响及机制。研究旨在为建立我国蔬菜食品的卫生安全预警体系的建立、评估有色金属矿区蔬菜砷生物有效性、减轻砷对人体健康安全风险提高科学依据及技术支撑。

Health risk from arsenic contaminated vegetables caused by mining activities is a serious issue in China, especially in Guangxi province, where problem of arsenic contamination in vegetables grown around the arsenic contaminated mining area has been largely reported. Bioavailability of arsenic is an important indicator to measure the arsenic toxicity and human health risk assessment. But the robust methods for arsenic bioavailability assessment is scarce, consequently few researches on arsenic bioavailability assessment in vegetables grown in arsenic contaminated mining area. The research goals of this project are to (1) establish an in vitro/ Caco-2 cell model to assess the arsenic bioavailability in vegetables (2) assess the arsenic bioavailability and human health risk in various vegetables grown around the arsenic contaminated mining area (3) investigate the effects of cooking methods on arsenic bioavailability in vegetables and its mechanism. The output results of this project will provide valuable scientific information for the establishment of food safety alert system, the appropriate adjustment of vegetable planting structure and cooking methods to minimize arsenic risk in human.