反转录转座子是动、植物基因组中最为丰富的一类转座元件，近年来成为基因.克隆、生物多样性及基因组进化研究的重要工具。本项目在已经从建鲤基因组中分离出Ty3-gypsy 反转录转座子反转录酶基因（RT 基因）片段的基础上，拟分离和鉴定建鲤基因组中具有转录活性的Ty3-gypsy 反转录转座子，并通过染色体步移技术获得其全长序列，在此基础上开发建立鲤的S-SAP 分子标记并转化为SCAR 标记技术，分析鲤鱼种群的遗传差异和种质资源。此外，将Ty3-gypsy 反转录转座子的启动子与GFP 基因构建为融合基因，通过分析GFP 蛋白的表达活性来解析建鲤基因组中Ty3-gypsy 反转录转座子启动子的活性。本项目旨在揭示反转录转座子在鲤鱼种群中的变异和遗传多样性及分析不同种群的遗传多样性，并筛选到与种群相关的分子标记。

Retrotransposons are Powerful tools for studies of gene cloning,biodiversity and Phylogeny due to their ubiquitous distribution， high copy numbers， a considerable degree of Sequence heterogeneity and irreversible insertion in plant and animal kingdom. In this study,based on isolating of RT sequence by degenerate primers aimed at RT conserved motifs, the different methods such as PCR and genomic walking were explored to identify LTR sequences and complete sequences of TY3-GYPSY retrotransposon from Cyprinus carpio genome.in addition,based on isolated LTR sequences ,the S-SAP technique of Cyprinus carpio will be established ,and this method will be used to analyse Genetic Diversity of Germplasm Resources on Cyprinus carpio.in the end, Study on the Activity of retrotransposons Promoter will be explored by the method that the fusion gene of retrotransposons Promoter and GFP was expressed in the in vitro expression system. The study will reveal the retrotransposon variation and biodiversity on Cyprinus carpio genome and get a molecular marks related to the Cyprinus carpio populations.