在自噬过程中，细胞内功能紊乱或冗余的生物大分子和细胞器回收至溶酶体中被降解。我们前期的研究中发现微丝纤维在自噬体分离膜双层膜结构内聚合，自噬体形成过程中CapZ等微丝相关蛋白参与自噬体分离膜中微丝纤维动态变化的调控。近年已有数篇文章报道了微丝纤维参与细胞自噬的相关研究，但是目前还没有关于自噬基因参与微丝纤维动态变化尤其是分支状微丝纤维动态变化的相关研究。自噬是应激条件下细胞内发生的生命过程，在非应激条件下，自噬相关蛋白与微丝相关蛋白之间的相互作用是否会对微丝骨架的动态变化进行调控是本项研究想要探讨的论题。在这项研究中我们将通过研究自噬相关基因敲除细胞在非应激条件下微丝纤维特别是分支状微丝纤维的动态变化筛选参与调控自噬相关基因，并通过进一步的研究阐明其影响微丝纤维骨架动态变化的分子机制，为自噬基因参与细胞膜泡运输，细胞迁移和膜重排等过程提供证据。

During autophagy, macromolecules and dysfunctional or redundant organelles were recycled to lysosome for degradation. Our studies have shown the actin scaffold assembles inside the central cavity of isolation membrane during autophagy, and some actin binding proteins like CapZ play roles in regulate branch actin network dynamics during this process. There have been several studies focused on the roles of actin filaments in autophagy recently, however there’s barely no report about the autophagy-related genes participate in actin dynamics regulation especially for the branch actin network. Autophagy is induced when cells undergo kinds of stress. Whether the interaction between autophagy proteins and microfilaments related proteins take part in the regulation of the assembly and disassembly of actin network is unknown. We highlight these non-autophagic roles of autophagy-related proteins with the aim of drawing attention to this growing, but unexplored, research topic. In this study, we will observe the difference of the autophagy related genes knockdown or knockout cells, and further demonstrate the mechanism of the regulation. This study will provide some evidence for autophagy related proteins play roles in modulation of vesicle traffic, cell migration and membrane reorganization.