循环肿瘤细胞适配子筛选及可逆捕获释放研究

循环肿瘤细胞是肿瘤相关研究领域的重要方向，对肿瘤早期诊断、预后评估及疗效监测等方面有重要的指导性意义。如何从循环肿瘤细胞含量极低的血液标本中，有效的提取出数量极少的目标，是研究者需要解决的核心问题。循环肿瘤细胞的识别、捕获与释放体系并不完善，这是其在临床上应用受限的一个重要因素。本研究以循环肿瘤细胞为靶标，拟运用细胞SELEX(cell-SELEX)技术筛选循环肿瘤细胞的核酸适配子；并进一步通过主客体相互作用发展可逆捕获释放研究。

The study of circulating tumor cells (CTCs) becomes more and more important in cancer relating research fields. Sensitive detection of CTCs paves a new way for tumor early diagnosis, prognosis and therapeutic monitoring . The researchers have to face the core issue and effectively extract very small quantity of CTCs from blood samples. To date, the identification, capture and release system targeting CTCs has not been well developed. Hence, this is an area of research that needs serious consideration. In this study, we aim to target CTCs, intend to use cell-SELEX for aptamer screening; and further develop reversible capture and release systems through host-guest interactions.