在橡胶树(Hevea brasiliensis)中，橡胶生物合成是以蔗糖为原料在特化的乳管细胞中进行，每次割胶后乳管细胞都需要消耗大量的蔗糖进行胶乳（乳管的细胞质）的再生，然而蔗糖的过度消耗却可能导致橡胶树死皮病的发生。前期研究发现，胶乳蔗糖合成酶可能在调控胶乳蔗糖代谢和防止胶乳蔗糖过度消耗方面起重要作用。本项目拟从转录和翻译水平研究胶乳蔗糖合成酶基因的表达特性，纯化胶乳蔗糖合成酶并了解其酶学特性，通过分子鉴定和生化特性研究，确定胶乳蔗糖合成酶的关键成员，明确其在调控胶乳蔗糖浓度水平方面所起的重要作用。项目结果有助于阐明橡胶树胶乳蔗糖代谢的调控机理，为全面揭示橡胶树胶乳蔗糖代谢与胶乳再生以及橡胶产量的密切关系奠定理论基础，为橡胶树产量定向分子改良提供重要的靶基因。

In rubber tree (Hevea brasiliensis), rubber is synthesized in the cytoplasm (latex) of highly specialized cells called laticifers use sucrose as the precursor molecule. After tapping, there need large amounts of sucrose for the regeneration of latex, while excessive sucrose consumption often result in the occurrence of tapping panel dryness (TPD). We found that Sus play an important role in preventing excessive sucrose consumption in the laticifers. In this study, the sucrose synthase were isolated and detected in latex basing on transcription and translation level, including gene and protein expression, gene function and characteristics of enzyme.The results will reveal how and which the key sucrose synthase gene regulate the sucrose metabolism in latex, and further regulate the rubber synthesis and the regeneration of latex. It is hoped to obtain one or more sucrose synthase genes that relate to rubber yield, provide important genes for molecular assistant breeding and transgene in Hevea.