海藻糖酶作为昆虫能量代谢和体内几丁质合成途径的关键酶，其基因表达情况和酶活性能否正常发挥直接关系到昆虫能否正常发育、蜕皮、变态和繁殖等。因此，Treh基因的功能及作为害虫控制靶标的研究具有重要的理论意义和应用价值。本项目拟以水稻害虫褐飞虱和赤拟谷盗为研究对象，采用RNAi技术和注射过量海藻糖分别制备Treh基因表达的下调和上调样品，利用高通量转录组测序结合数字表达谱（DGE）及同位素标记相对和绝对定量（iTRAQ）技术分析筛选获得Treh基因调控下游的所有网络通路，然后利用褐飞虱验证上述筛选结果，并在褐飞虱和赤拟谷盗活体中鉴定Treh基因调控的核心网络通路，最后明确Treh基因对几丁质合成通路、能量代谢通路和其它关键网络通路的调控功能，评价Treh对其它非靶标昆虫的安全性。该项目不仅可在理论上阐明Treh基因的功能，明确Treh基因的控害机理，而且可评估Treh基因作为害虫控制靶标的潜力。

The expression and enzyme activity of trehalase, essential for insect energy metabolism, which is the first key enzyme in chitin synthesis pathway, directly influence the normal development, molting, metamorphosis and reproduction. So the research of trehalase function and its role as pest control target is of considerable theoretical significance and practical value. In this project, Tribolium castaneum and brown planthopper as object, RNA interference and excessive trehalose injection are employed to prepare the Treh down-regulated and Treh up-regulated samples for Digital Gene Expression (DGE)tag profiling and Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technology to screen all pathways controlled by Treh. Then brown planthopper are used to confirm the above selected results and Tribolium castaneum and brown planthopper will be used to identify the core pathway regulated by Treh to clearly define the Treh regulatory functions of chitin synthesis, energy metabolism as well as other key pathways and evaluate the security to non-targeted insects.In addition to clarifying the function and pest control mechanism of Treh gene, the project can assess the potential of Treh gene as target for pest control.