混合样本分析在法医实践中的需求日益增多。然而，由于混合样本组分分离困难，STR分型结果解读复杂，传统标记数量有限，新型标记研究不足等原因，混合样本分析经常不能获得明确鉴定意见，导致该类检材无法充分发挥其证据价值，成为法医物证鉴定难题之一。近年来，大规模并行测序技术（Massively parallel sequencing, MPS）在深度读取序列信息的同时可以完成多类型遗传标记、多样本的并行检测，为混合样本分析提供了新的技术手段。课题组前期应用该技术对多态性遗传标记的研究也显示了其在混合样本分析中具有良好的应用前景。本项目拟充分利用传统毛细管电泳和新技术MPS的优势，探索各类遗传标记（STR、SNP、STR-SNP、Microhaplotype）在混合样本分析中的贡献，建立有效的检测体系及科学的分析方法，最终为混合样本分析提供新的解决手段。

Mixture analysis is an ever-increasing need for forensic application. However, with the mixed component, complex STR profiles, limited genetic candidates and unsophisticated study of new markers, mixture analysis become a key scientific issue that is of high priority. And abovementioned problems make mixtures become ineffective evidence sometimes. In recent years, massively parallel sequencing (MPS) provides prospect for the solution of this scientific problem in terms of technical feasibility. MPS renders possible the simultaneous testing of different genetic markers and different samples. Our pilot study of polymorphism genetic markers with MPS demonstrated the prospect of this technology for mixture analysis. This whole study aims to explore various genetic markers of STR, SNP, STR-SNP and Microhaplotype with traditional capillary electrophoresis (CE) and new technology of MPS for mixture analysis and establish effective detection system and scientific analysis methods, providing new solution for mixture sample analysis.