拟南芥EIN3/EIL1与FLD互作介导乙烯推迟开花的分子机制
结题报告
批准号:
31970534
项目类别:
面上项目
资助金额:
58.0 万元
负责人:
陶增
依托单位:
学科分类:
表观遗传调控
结题年份:
2023
批准年份:
2019
项目状态:
已结题
项目参与者:
陶增
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中文摘要
花期转换是植物由营养生长进入生殖生长的重要标志,受到多种内部和外部因素的影响,包括激素。乙烯是一种植物气体激素,乙烯处理可以推迟植物开花,但具体分子机制有待进一步阐述。FLD是拟南芥开花自主途径中的一个重要基因,通过催化组蛋白去乙酰化和去甲基化抑制FLC的表达。本项目通过FLD为诱饵筛选文库,得到候选互作蛋白为乙烯信号关键调控因子EIN3及EIL1。进一步证实,EIN3/EIL1可以在体内与FLD互作,ein3eil1表现推迟开花,推测可能与FLD介导组蛋白修饰调控FLC的表达有关。初步结果表明,乙烯处理推迟开花的过程依赖于FLC的表达,EIN3和EIL1可以结合到FLC的染色质上,ein3eil1中FLC位点H3K4me2的修饰水平上升。申请人拟通过本申请,深入分析乙烯处理推迟开花的分子机制,揭示组蛋白修饰在介导乙烯信号与花期转换调控中的作用,为植物激素信号转录调控的理解提供新的思
英文摘要
Floral transition is an important development switch from vegetative stage to reproductive stage in plants which affected by various internal and external factors including hormone. Ethylene is a plant gas hormone. The treatment of ethylene can delay flowering time in Arabidopsis but the molecular mechanism was largely unknown. FLD is an important gene of autonomous pathway in flowering regulation, which catalyzed histone deacetylation and demethylation to suppress FLC expression. Screening library with yeast two hybrids, EIN3 and EIL1, key factors in the ethylene signal pathway, were identified as FLD partners. Furthermore EIN3 and EIL1can interact with FLD in vivo, implied that the delay-flowering phenotype of ein3eil1 maybe associate with histone modification to suppress FLC which mediated by FLD. Preliminary data proved that delay-flowering under ethylene treatment was FLC-dependent. On the one hand EIN3/EIL1 could bind to FLC chromatin, the other hand the level of H3K4me2 in the FLC locus was increased in eil3eil1. Based on these results, my project will propose to reveal the molecular mechanism of delay flowering with ethylene treatment, elucidate the important roles of histone modification in the regulation of ethylene signal and floral transition. I believe that our results would develop the novel understanding with ethylene signal transduction and transcriptional regulation, also explore potential targets to utilize the hormone.
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DOI:https://doi.org/10.1093/plphys/kiad131
发表时间:2023
期刊:Plant Physiology
影响因子:--
作者:Mengting Xu;Xiaoxiao Li;Wei Xie;Chuyu Lin;Qiannan Wang;Zeng Tao
通讯作者:Zeng Tao
DOI:10.1007/s44297-023-00003-y
发表时间:2023-08
期刊:Crop Health
影响因子:--
作者:Zeng Tao;Fei Yan;M. Hahn;Zhonghua Ma
通讯作者:Zeng Tao;Fei Yan;M. Hahn;Zhonghua Ma
N1617介导稻瘟病菌兼性异染色质形成的分子机制
  • 批准号:
    32370200
  • 项目类别:
    面上项目
  • 资助金额:
    50万元
  • 批准年份:
    2023
  • 负责人:
    陶增
  • 依托单位:
稻瘟病菌效应蛋白转录重编程的分子机制
  • 批准号:
    --
  • 项目类别:
    面上项目
  • 资助金额:
    58万元
  • 批准年份:
    2021
  • 负责人:
    陶增
  • 依托单位:
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