拟南芥CERK1是参与真菌几丁质(chitin)信号识别及转导的关键受体激酶。为研究CERK1功能复合物中的成员，我们前期通过免疫沉淀与质谱分析，鉴定到腈水解酶NIT蛋白能与CERK1发生蛋白互作，chitin可诱导二者结合增强。通过CRISPR技术我们获得了nit1 nit2 nit3三突变体，在生理和生化水平上证实NIT1、NIT2和NIT3在IAN合成IAA的过程中发挥冗余功能。有趣的是，NITs可在拟南芥对灰霉菌抗性应答中发挥正调控作用。为深入研究NITs的功能及其与CERK1的调控关系，本项目拟首先从生理功能及分子层面确证NITs介导的IAN合成IAA代谢途径直接参与了植物病原防御。进一步，通过CERK1对NITs功能调节的机制研究，以及NITs对CERK1的反馈调节机制的解析，探讨激素代谢途径与膜受体激酶CERK1介导的PTI信号通路之间的功能关系。

CERK1 is a key receptor kinase involved in the recognition and transduction of fungal chitin signals in Arabidopsis. In order to study the members of functional CERK1 receptor complex, we identified the interaction proteins by IP and HPLC-MS. We found that nitrilase NIT1 interacts with CERK1 and the interaction is enhanced by chitin signaling. In addition, we obtained the nit1 nit2 nit3 mutants by CRISPR technology. At the genetic and physiological level it was confirmed that NIT2/NIT1/NIT3 plays an important physiological role in IAA synthesis metabolized from IAN. Moreover, studies show that NITs play a positive regulatory role in Arabidopsis response to Botrytis cinerea. In order to further study the function and regulation mechanism of NITs involved in pathogen response, we firstly verify the physiological function and detect the molecular mechanism of of NITs involed in pathogen defense. Moreover, we will explore its functional relationship between CERK1 mediated PTI response and auxin metabolism pathway mediated by NITs.