The Zygotic Division Regulator ZAR1 Plays a Negative Role in Defense Against Botrytis cinerea in Arabidopsis.
The Zygotic Division Regulator ZAR1 Plays a Negative Role in Defense Against Botrytis cinerea in Arabidopsis.
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DOI：
10.3389/fpls.2021.736560
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发表时间：
2021
影响因子：
5.6
通讯作者：
Liu B
中科院分区：
文献类型：
--
作者：
Chen L;Xiao J;Song Y;Li Y;Liu J;Cai H;Wang HB;Liu B
几丁质激发子受体激酶1(CERK1)酪氨酸428位的磷酸化/去磷酸化循环在几丁质诱导的拟南芥免疫中起着至关重要的作用。在这项研究中，我们使用差异多肽下拉试验(PPD)来确定可能参与该周期下游的因素。我们鉴定了合子停滞1(ZAR1)，并表明当CERK1的酪氨酸428(Y428)残基被去磷酸化时，它与CERK1特异地相互作用。ZAR1最初被认为是拟南芥中钙调素和G蛋白信号的整合者，以调节合子分裂。我们目前的结果表明，ZAR1也对灰霉病菌的防御起到了负面作用，并且与其同系物ZAR2在这一过程中起到了多余的作用。Zar1-3zar2-1双突变体对灰霉病的抗性高于zar1-3单突变体、zar2-1单突变体和野生型植株。此外，在Zar1-3zar2-1双突变体中，许多诱导表达的防御反应基因在灰霉病菌侵染后表达增加，这与ZAR蛋白在防御反应中的抑制作用是一致的。因此，我们的发现为深入了解ZAR1在多种防御和发育调节途径中的功能提供了依据。
A phosphorylation/dephosphorylation cycle at tyrosine 428 of CHITIN ELICITOR RECEPTOR KINASE 1 (CERK1) plays an essential role in chitin triggered immunity in Arabidopsis thaliana. In this study, we used a differential peptide pull-down (PPD) assay to identify factors that could participate downstream of this cycle. We identified ZYGOTIC ARREST 1 (ZAR1) and showed that it interacts with CERK1 specifically when the tyrosine 428 (Y428) residue of CERK1 is dephosphorylated. ZAR1 was originally characterized as an integrator for calmodulin and G-protein signals to regulate zygotic division in Arabidopsis. Our current results established that ZAR1 also negatively contributed to defense against the fungus Botrytis cinerea and played a redundant role with its homolog ZAR2 in this process. The zar1-3 zar2-1 double mutant exhibited stronger resistance to B. cinerea compared with zar1-3 single mutant, zar2-1 single mutant, and wild-type plants. Moreover, the inducible expression of numerous defense response genes upon B. cinerea infection was increased in the zar1-3zar2-1 double mutant, consistent with a repressive role for ZAR proteins in the defense response. Therefore, our findings provided insight into the function of ZAR1 in multiple defenses and developmental regulation pathways.
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影响因子：
64.8
作者：
Cheng, Zhenyu;Li, Jian-Feng;Niu, Yajie;Zhang, Xue-Cheng;Woody, Owen Z.;Xiong, Yan;Djonovic, Slavica;Millet, Yves;Bush, Jenifer;McConkey, Brendan J.;Sheen, Jen;Ausubel, Frederick M.
通讯作者：
Ausubel, Frederick M.
影响因子：
30.3
作者：
Liu, Jun;Liu, Bing;Wang, Jinfa
通讯作者：
Wang, Jinfa
影响因子：
64.8
作者：
Chinchilla, Delphine;Zipfel, Cyril;Boller, Thomas
通讯作者：
Boller, Thomas
DOI：
10.1073/pnas.0705147104
发表时间：
2007-12-04
影响因子：
11.1
作者：
Miya, Ayako;Albert, Premkumar;Shibuya, Naoto
通讯作者：
Shibuya, Naoto
影响因子：
7.4
作者：
Liu, Jinman;Ding, Pingtao;Zhang, Yuelin
通讯作者：
Zhang, Yuelin