基于多组学分析探索植物核纤层类似蛋白影响染色质状态和基因表达的动态调控规律

基因表达调控是高度复杂又精细的，与细胞核内的染色质状态和空间定位密切相关。核纤层连接核膜与染色体，不仅为细胞核提供结构稳定性，而且调控三维染色质结构和基因表达。相比于医学和动物领域的核纤层研究，植物领域的研究还远远不足。我们通过基因网络功能预测和突变体表型鉴定，已初步发现KAKU4等核纤层类似蛋白可能影响叶片衰老、开花早晚、光/温反应、昼夜节律等过程。我们将利用组学大数据整合与解析的研究体系和分析平台，紧密结合实验验证，探索植物（拟南芥和水稻等）核纤层类似蛋白对染色质状态和基因表达的动态调控规律，挖掘重要功能模块、关键调控节点、RNA可变剪切形式、蛋白修饰位点等相关信息，构建植物核纤层类似蛋白影响基因表达的调控网络和动态图景。总之，本课题瞄准核纤层调控基因表达这一前沿科学问题，运用系统生物学研究手段，以期把植物核纤层类似蛋白的研究提升到一个更系统的层面，这将具有重要的理论意义和应用价值。

Gene expression regulation is highly complex and delicate, which is closely related to chromatin states and spatial localization in the nucleus. The nuclear lamina links the nuclear membrane with chromosomes, which not only provides structural stability for the nucleus, but also plays a key role in regulating three-dimensional chromatin structure and gene expression. Compared with the study of nuclear lamina in medical and animal field, the related study in plant field is still limited. Through gene network function prediction and mutant phenotype identification, we have preliminarily found that nuclear lamina-like proteins such as KAKU4, might affect the biological processes of leaf senescence, flowering time, light/temperature response, circadian rhythm and so on. We will conduct the infrastructure system and analysis platform of the integration and analysis for omics big data, together with experimental verification, to study the molecular mechanisms about how lamin-like proteins dynamically regulate the chromatin states and gene expression in plants (Arabidopsis thaliana and rice, etc.). We will discover the important functional modules, key regulatory nodes, variable splicing isoforms and other related information. Furthermore, we will construct plant lamin-like protein related regulatory network and dynamic landscape. In a word, this research topic focuses on the frontier in the study of nuclear lamina-mediated regulation function. By means of systems biology, we might improve the understanding of plant lamin-like proteins affecting gene expression to a more systematic level, which will have important theoretical significance and application value.