苜蓿（Medicago L.）花的颜色是由基因控制且是稳定的，控制花色的基因是多效的且是连锁的，不同花色苜蓿变异类型是不同苜蓿种之间杂交的过渡类型，基于上述观点，项目以花色为研究对象，研究白花苜蓿的分类学地位问题，构建DNA指纹图谱；黄花苜蓿-紫花苜蓿和白花苜蓿三者之间杂交的中间过渡类型；对不同花色苜蓿数字化描述（ID值）；对无性系进行生育期、植物学、农艺学、品质等特性评价，筛选所属特殊性状；通过分子标记技术，筛选DNA差异片段，对差异片段进行基因功能预测，通过与上述大田研究指标比对，筛选与不同花色苜蓿特有性状相关的控制基因。苜蓿花色从黄色到深紫色（白花苜蓿），基本上涵盖了苜蓿花色的变异范围，对于白花苜蓿分类学、中间过渡类型、苜蓿核心种质的研究构建将起到积极作用；为苜蓿在抗逆性、高产、品质等研究等方面提供依据，为育种奠定基础；通过对基因功能预测将阐明不同花色苜蓿所具有不同特质性状的机理。

The flower colors of alfalfa（Medicago L.） with stable heredity are controlled by genes which are multi-functional and linked. Intermediate forms of alfalfa due to the hybrids among different species. In the future research，the experiments will be conducted including taxonomy of alfalfa with white flower、DNA finger prints 、intermediate forms and their accurate description（ID）. The clones of different color will be compared in growing period、botany、agronomy、quality to characterize the difference and speciality. Using molecular marker technology，distinctive DNA fragments will be searched for the genetic function prediction which control the phenotypic characteristics. Floral color variation in alfalfa from oyster to deep purple which basically covers the range will be beneficial to the taxonomy of alfalfa with white flower 、ntermediate forms 、core collection 、 alfalfa . The evaluation to different color alfalfa will become a strong foundation for stress resistance、high yield、quality etc. Molecular maker will be beneficial to reveal the mechanism why different flower color with different properties.