免疫耐受分子HLA-G与肿瘤发生发展密切相关。HLA-G mRNA编码产生7种异构体分子（HLA-G1～-G7）。我们研究发现，HLA-G1表达与卵巢癌细胞成瘤及转移能力直接相关，但HLA-G1～-G7异构体在卵巢癌肿瘤免疫中尚缺乏体外及荷瘤动物模型系统比较研究。本项目在前期研究基础上，以卵巢癌细胞HO-8910及临床病例为研究对象，系统分析、比较HLA-G1～-G7分子在卵巢癌细胞上表达前后对免疫细胞功能、肿瘤细胞迁徙及侵袭能力的变化；通过Balb/c裸鼠，建立分别表达HLA-G1～-G7异构体分子的卵巢癌荷瘤模型，分析7种不同HLA-G异构体分子对肿瘤细胞成瘤能力、脏器转移及小鼠生存时间的影响；通过卵巢癌病例，分析HLA-G异构体mRNA及蛋白表达与卵巢癌患者临床特征及预后的关系，阐述特定HLA-G异构体分子在卵巢癌发生发展中的作用及其机制，为肿瘤生物学治疗提示新的理论基础和实践探索。

The induced expression of the immunotolerant HLA-G were observed in various malignancies and play critical roles in the pathogenesis and progression of the diseases. Due to its primary transcript alternative splicing, seven isoforms were produced (HLA-G1～HLA-G7). Our previous study showed that the HLA-G1 isoform expression was strongly associated with the HO-8910 ovarian cancer cell line tumorigensis and invasiveness. However, a sysmetic evaluation the significance of all seven HLA-G isoforms remains to be explored in ovarian cancer tumor immunology. In this study, we cloned and expressed the HLA-G1～HLA-G7 isoforms in the HLA negative above mentioned HO-8910 ovarian cancer cell line, respectively. With these HLA-G transfectants, a systmatic analysis will be performed to explore the roles of each specific HLA-G isoform on the T and NK cell cytotoxicity and the profile of cytokine production; roles of HLA-G isofrom on the capability of the tumor cell HO-8910 migration and invasiveness in vitro. Furthermore, a mouse tumor model with these HLA-G transfectants with Balb/c，nu/nu mice will be established to explore the roles of each HLA-G isoform on the tumor development, metastasis and mouse survival.Taken together, this study would provide further valuble understanding of significnace of HLA-G isofroms in tumor immunology.