侧脑室外侧壁的脑室下带（SVZ ）是脑内具有再生能力的区域之一，我们的前期研究表明脑出血可激活SVZ细胞再生，再生的SVZ细胞可向损伤区域迁移。然而脑组织通过这种内源性神经细胞再生而获得的自我修复能力是非常有限的，不足以改善缺失的神经功能。我们试图寻找影响脑出血后内源性神经细胞再生的因素，来增强内源性神经细胞的再生能力。凝血酶在脑出血后内源性神经细胞再生中发挥重要作用，我们的前期研究表明小剂量凝血酶预处理（TPC）可减轻随后脑内注射大剂量凝血酶或脑出血引起的脑损伤，但小剂量TPC对内源性神经细胞再生产生何种影响目前尚不明确。因此本研究拟采用时间间隔成像系统在活体脑片上动态追踪SVZ细胞，以及应用免疫荧光双标技术、荧光金逆行标记技术结合细胞内微电极记录技术，探讨TPC对脑出血后SVZ细胞再生、迁移、分化及整合至神经环路的影响，为脑出血后激发内源性神经细胞再生能力的研究提供新思路。

The subventricular zone (SVZ) of the lateral ventricle is one of regions possessing regeneration potentiality in the brain. Our studies have indicated intracerebral hemorrhage can induce SVZ regeneration and regenerated SVZ can migrate to the injured area. However, self-repair capacity acquired by brain tissues by endogenous neurogenesis is very limited and not enough to ameliorate neurological deficit. We attempt to enhance endogenous neurogenesis by exploring factors that affect endogenous neurogenesis. Recent studies have showed thrombin plays an important role in the endogenous neurogenesis after intracerebral hemorrhage. Our studies have showed thrombin preconditioning at a small dose can attenuate injury induced by thrombin at a large dose or intracerebral hemorrhage. However, the effect of thrombin preconditioning at a small dose on endogenous neurogenesis is uncertain. Therefore, this study plans to dynamically trace SVZ cells in living brain slices by time-lapse microscopy and to explore the influence of TPC at a small dose on SVZ cells regeneration, migration, differentiation and integration into the existing brain circuitry after cerebral hemorrhage by immunofluorescent double labeling, confocal microscopy, retrograde labeling of fluorogold and intracellular microelectrode recording methods, aiming to provide novel ideas for activation of endogenous neurogenesis after intracerebral hemorrhage.