上皮间质转化（EMT）是非小细胞肺癌（NSCLC）出现转移、耐药最终导致患者死亡最重要的原因，抑制肿瘤细胞的上皮间质转化及EMT的信号传导通路是治疗非小细胞肺癌的重要策略。清热解毒法是中医肿瘤的重要法则.在治疗非小细胞肺癌的疗效的证治规律和循证医学的Meta分析表明清热解毒法能有效抑制NSCLC化疗期间出现的上皮间质转化,但其作用机制不明。课题组在已经构建上皮间质转化调控蛋白靶标-药物分子数据库的基础上，借助分子对接技术筛选出清热解毒法对上皮间质转化干预作用的靶点，采用PET-CT技术结合免疫组化、Western blot和real-time PCR等实验方法，实时、动态、精确的检测药物对NSCLC上皮间质转化的干预作用,从核酸、蛋白水平验证研究清热解毒法调控EMT的靶点和信号通路。为应用清热解毒法治疗NSCLC提供实验证据和理论支持，具有重要的科学价值和临床指导意义。

Epithelial mesenchymal transition (EMT) is the most critical factor led to non-small cell lung cancer (NSCLC) transfer, resistance and eventually led to the death. Inhibition of EMT activation signal conduction in tumor cells has become an important strategy in the treatment of non-small cell lung cancer. Treatment of law and evidence-based medicine, Meta-analysis results for Qingrejiedu drugs to treat non-small cell lung cancer show that the Qingrejiedu method can effectively inhibit NSCLC's epithelial mesenchymal transformation.during chemotherapy. However, its mechanism of action and means is not clear. On the basis of the preliminary study , Innovatively our group use PET-CT technology,real-time, dynamic, accurately,to detect the drug's intervention effect of the NSCLC epithelial -mesenchymal transformation. And base on the database of the regulatory proteins target - drug molecules of epithelial mesenchymal transition , with a molecular docking ,we screen the Qingrejiedu method's interventing targets in the role of epithelial mesenchymal transition. And then by immunohistochemistry, Western blot and real-time PCR experiments to validate the targets and means in controling the EMT for Qingrejiedu method from the nucleic acid and protein levels. The results provide experimental evidence for the clinical application of Chinese medicine treatment of NSCLC, and further theoretical support from a new perspective, and has important scientific significance and potential applications.