秀丽隐杆线虫FAR基因在放射损伤和修复过程中的作用及分子机制研究

放射治疗是肿瘤治疗的主要手段之一，是利用电离辐射的作用使肿瘤组织损伤或死亡。在放疗过程中，放射线在杀灭肿瘤细胞的同时，也会损伤正常组织，这一副作用极大的影响了肿瘤病人的术后恢复和生存状况。维生素A具有放疗增敏和保护正常细胞的作用，而多不饱和脂肪酸对肿瘤细胞具有一定抑制作用。维生素A和多不饱和脂肪酸在肿瘤发生发展和治疗过程中发挥着重要的作用，但其具体功能和机制尚未阐明。线虫FAR蛋白具有结合和利用维生素A和脂肪酸的功能，我们之前的研究发现FAR基因能够促进线虫的生殖和发育，但该基因在DNA损伤修复中的作用机制尚待研究。本项目应用秀丽隐杆线虫模型筛选放射后差异表达基因，研究其功能和分子机制；结合线虫FAR基因的功能，研究维生素A和脂肪酸在放射损伤及修复过程中的作用机制。本课题的完成将为放射后细胞损伤修复分子机制的研究提供新的思路，为提高放疗效果和放疗后恢复提供重要的理论依据。

Radiotherapy is a main technique to cure tumour. Ionization radiation of radiotherapy injures tumour issue even induces the tumour issue death. During the process of radiotherapy, ionization radiation kills tumour cells with injuring healthy issue and this side-effect influence cancer patients’ postoperative recovery and living situation significantly. Retinol (Vitamin A, VA) increase radiosensitivity and protect healthy cells without injuring. Polyunsaturated fatty acids (PUFA) suppresses tumour cells functionally. VA and PUFA play an improtant role in the development process of tumour and therapeutic process, but specific function and mechanism are still unclear. FAR in Caenorhabditis elegans has functions of binding and utilizing VA and PUFA, and we found FAR could promote reproduction and development of C. elegans, but the effect of FAR in DNA repairing mechanism is still unclear. This project select differential expressed genes induced by ionization radiation by using C. elegans as model, and research the gene function and molecular mechanism; We study the effect of VA and PUFA on repairing ionization radiation damage by utilizing FAR. Our research will provide new ideas in molecular mechanism study of ionization radiation damage and theoretical foundation of improving radiotherapy results and postoperative recovery.