运用基因工程技术改造、提高微藻产油效率是当前人工制备生物柴油技术中亟待解决的重要问题。由于以往对藻类油脂合成代谢机制以及控制脂质积累的关键因素了解不够，从而限制了微藻产油技术的大规模开发应用。本研究拟选取产alkenone型脂质的典型微藻（Tisochrysis lutea）为主要研究对象，运用多技术体外表达纯化脂滴蛋白、制备抗体并通过免疫电镜对主要蛋白进行定位，分析确定脂滴的原生蛋白；结合分子生物学技术查明原生蛋白的基因转录和蛋白表达水平，及其与脂质积累的相关性从而确定这些蛋白的主要功能。在此基础上，运用ESI-MS/MS技术对脂滴进行脂组学研究，结合主要蛋白的功能分析，以期揭示alkenone型脂质的代谢途径。从而为通过基因工程提高微藻油脂产量、优选藻种提供理论依据和关键信息。

To increase the productive rate of lipids in microalgae through genetic manipulation is one of the most concerned problems in microalgae biodiesel production. Due to insufficient understanding on lipid metabolic mechanisms and the key factors controlling lipid accumulation, large scaled development and utilization of microalgae biodiesel have been handicaped. The proposed study will focus on the metabolic pathway of alkenone-producing microalgae, with Tisochrysis lutea as target species. Using immuno-electron microscopy the major proteins from lipid droplets will be located through the antibodies generated against them. Then the function of those confirmed proteins in lipid droples will be revealed through their gene transcript and protein expression levels during lipid accumulation. Combined with lipidomics analyses of lipid droplets, the possible metabolic pathway of alkenone can be properly established. Thus, this study will provide theoretical basis and important technologic information for improving lipid production in microalgae and help to optimize algae species selection.