lncRNA表达具有组织时序性，可发挥miRNA海绵或调控基因转录、翻译等功能，在多种疾病中发挥重要作用，但在移植肝损伤时的表达和作用机制尚不明确。前期芯片筛选和移植肝组织验证发现lncRNA-neat1呈高表达。下调neat1后，可抑制缺氧/复氧时的肝细胞损伤，同时发现miR-107表达上调和P53表达下调。生物信息学预测其可通过ceRNA方式结合miR-107调控P53/BECN1表达，但具体机制仍待探究。本课题拟通过体内、外实验揭示neat1、miR-107和P53/BECN1之间的调控作用，阐明neat1充当miR-107海绵调控P53/BECN1，从而影响移植肝损伤的分子机制。从组织和血浆水平，评价lncRNA-neat1/miR-107调控P53/BECN1/信号途径对移植肝损伤的临床意义，为其防治提供新思路。

Long noncoding RNAs (lncRNAs) exhibit tissue/developmental-stage-specific expression and can act as microRNA sponge or regulate gene transcription, translation to play important roles in various diseases. However, their expression and roles in the development of livergraft injury are still unclear. We have shown that lncRNA-neat1 is downregulated via microarray screening and tissue redetection. Moreover, downregulation of neat1 in hepatic cell line can significantly attenuate cell injury in hypoxia and reoxygenation condition, increase miR-107 expression and reduce P53/BECN1 expression. Bioinformatic analysis indicated that neat1, acting as miR-107 sponge to regulate P53/BECN1 expression, plays roles in the development of livergraft injury. Nevertheless, the detailed mechanisms still need to be illuminated. This study will reveal regulatory relationships among lncRNA-neat1, miR-107 and P53/BECN1, and clarify the mechanism of neat1 serving as miR-107 sponge to regulate P53/BECN1 expression in vitro or in vivo. Meanwhile, we will evaluate their clinical significances in tissue and plasma levels. This study would provide novel insights into the prevention and treatment of livergraft injury.