类风湿性关节炎(RA)是造成我国人群丧失劳动力和致残的主要疾病之一。自噬对RA病情的发展起关键作用。抑制PI3K/Akt/mTOR信号通路可诱导细胞自噬，促进滑膜细胞凋亡，抑制细胞增生。本项目以RA动物模型(AA大鼠)为受试对象，采用电针治疗，运用光镜、放免、免疫组化、RT-PCR、Western-blot 等检测手段，通过对滑膜组织形态结构、炎性细胞因子、生长因子、滑膜细胞P13K、Akt、mTOR蛋白表达、滑膜细胞自噬体的形成、自噬体膜标志性蛋白质、自噬相关基因Beclin-1、ULK1、Atg13的ｍRNA表达水平及胞浆内凋亡抑制因子c-IAP1、c-IAP2、Bcl-2、凋亡蛋白Caspase3/6/9表达的检测，研究电针对RA大鼠滑膜细胞自噬的影响，探索电针是否通过抑制PI3K/Akt/mTOR信号通路诱导细胞自噬，抑制滑膜细胞增生和抗炎的机制。

Rheumatoid arthritis (RA) is one of the major diseases that cause loss of labor and disability. PI3K/Akt/mTOR signal pathway regulating autophagy plays a key role in the development of RA. Inhibition of PI3K/Akt/mTOR signal pathway can induce cell autophagy, promote apoptosis of synovial cell , inhibit cell proliferation.This project uses the animal model of RA (AA rats) as subjects,uses electroacupuncture treatment,uses the light microscopy, radioimmunoassay, immunohistochemistry, RT-PCR and Western-blot and other detection .Through the detection of the expression of synovial tissue morphological structure,inflammatory cytokines, Growth factors、P13K、Akt、mTOR protein expression, synovial cell autophagosome formation, autophagic membrane marker proteins, ｍRNA expression of autophagy-related genes Beclin-1、ULK1、 Atg13，the expression of apoptosis inhibitory factor c-IAP1、c-IAP2、Bcl-2 and apoptotic protein Caspase3/6/9，to study the effect of electrocution on autophagy of synovial cell in RA rats,and to explore whether electroacupuncture can promote apoptosis and anti-proliferation of RA synovial cells by inhibiting PI3K/Akt/mTOR signal pathway regulating autophagy