脑细胞外微环境（brain extracellular microenviroment，BEM）损伤属中医“脑络损伤”，是缺血性中风的主要病理过程，气虚血瘀为主要病机，益气活血为主要治法。调节BEM有望成为脑缺血治疗新靶点。前期结果显示黄芪甲苷和三七总皂苷配伍抗脑缺血作用环节与BEM调控机制密切相关。BMSCs可靶向修复神经功能，受BEM调节，BMSCs-exosomes为其主要活性物质。故本项目以MCAO模型大鼠为研究对象，BEM组成单位脑细胞外间隙、细胞间液及细胞外基质为切入点，从整体动物实验研究组分中药促BMSCs-exosomes脑缺血后神经修复作用；并建立BEM体外模型，验证其作用机制与修复缺血后BEM，促进BMSC-exosomes分泌、存活及功能蛋白的释放有关。本项目可望丰富中医药“益气活血、从络论治”治疗脑缺血性疾病的实验依据，为促进脑缺血后神经功能恢复提供有效的防治方法。

Brain extracellular microenviroment (BEM) damage, namely”brain collaterals Impairmentin” traditional Chinese medicine (TCM), is the major pathological process of ischemic stroke. Qi deficiency and blood stasis are the principle pathogenesis of BEM damage, and tonyfying Qi and activating blood are their main therapy. Our Previous results showed that the anti-ischemic effect of compatibility of astragaloside and panax notoginseng saponins is closely related to regulating mechanism of BEM. BEM, as a potential target, could regulate BMSCs for targeting repairing neurological function. BMSCs-exosomes are the main active substances of BMSCs. In our project, a local cerebra ischemia rats model is as the study objective, the BEM constitutes unit of extracellular space, intercellular fluid and extracellular matrix can as entry points. The effective components of Huangqi and Sanqi compatibility in promoting BMSCs-exosomes to nerve regeneration after stroke are to studied in vivo experiments. Based on the above experiments, building the BEM model in vitro is aiming to explore the mechnism of TCM constituents reparing BEM and promoting BMSCs-exosomes after ischemia. The results of this project are to potentially enrich basis of TCM in treating cerebral ischemic diseases from”Tonyfying Qi and activating blood ,Regulating brain collateral”,and provide preventive methods for promoting the recovery of neurological function after cerebral ischemia.