实现骨质疏松(PMO)状态下牙周组织再生,对伴有PMO的牙周炎患者的临床治疗具有重要意义。课题组前期研究发现PMO不仅影响牙周组织的再生,还可降低骨髓基质细胞(BMSCs)的成骨能力。牙骨质蛋白1(CEMP1)是一种新发现的在骨及牙骨质再生中发挥重要作用的细胞因子。据此，我们设想利用基因强化型组织工程技术将CEMP1基因修饰的非骨质疏松状态的BMSCs进行同种异体移植将有望最大程度地实现PMO状态下牙周组织的再生。为明确PMO状态下宿主免疫系统对BMSCs同种异体移植的影响,本研究拟通过体外培养PMO状态下机体固有免疫的重要组成--巨噬细胞，并与CEMP1基因修饰的同种异体BMSCs共培养,探讨两者间的相互作用。在此基础上,通过动物实验明确CEMP1基因修饰的工程化复合物同种异体移植对PMO状态下牙周组织再生的作用,并探讨移植过程中机体免疫系统的调节机制。

Postmenopausal osteoporosis (PMO) is a systematic bone metabolism related disease that affects periodontal regeneration. PMO not only can promote the absorption of alveolar bone in periodontitis, but also can affect the regeneration of periodontal tissue. CEMP1 is a key cytokine for bone and cementum regeneration. Our previous studies have demonstrated that dysfunctional BMSCs are major contributors to the progression of osteoporosis. Hence, we hypothesize that allogeneic BMSCs modified by CEMP1 gene are expected to enhance the periodontal regeneration with osteoporosis. To explore the potential mechanism of immune response on allogeneic gene modified BMSCs, we would over express CEMP1 gene in rat BMSCs by using adenovirus vectors and checked the subsequent osteogenic effects. Next, macrophage would harvest from the ovariectomized (OVX) and Sham rats and co-culture with allogeneic gene modified BMSCs. Finally, periodontal defect and critical size bone defect would be carried out in OVX rats to investigate the effect of periodontal regeneration and the immunoreaction by using BMSCs modified by CEMP-1 gene.