本项目以纳米材料研究开发成为热点以及日益严峻的海洋环境污染为研究背景。针对目前关于海洋环境中的纳米碳60与其他污染物相互作用所产生的潜在生物毒性效应与机制的研究空白，拟以海洋模型生物贻贝为研究对象，分析纳米碳60与有机污染物苯并芘、重金属污染物铜在贻贝各组织中的生物蓄积，阐释海洋中的纳米碳60产生“木马效应”转运污染物进入生物体的潜在机制。同时，应用独特的生物个体、组织、细胞以及分子的多水平生物标记物分析体系，阐明海洋环境中的纳米C60与污染物相互作用的多水平复合生物毒性效应与机制，为评估纳米材料在海洋污染条件下所产生的生物致毒效应以及预测通过食物链对人体所产生的健康威胁提供理论支撑，为调控纳米材料的可持续性开发应用提供科学依据。

Compounds from the nanotechnology industry, such as carbon-based nanomaterials, are strong candidates to contaminate aquatic environments because their production and disposal have exponentially grown in a few years. Fullerene C60 and other classic environmental pollutants in the aquatic environment can induce complex detrimental impacts on aquatic organisms. Integrated biomarkers at different levels of biological organizations are designed and applied to analyze such impacts on marine mussels following exposure to selected environment pollutants (Fullerene C60, BaP, Cu). Chemicals will be quantified in dissected tissues to indicate the alterations of distribution and bioaccumulation after BaP/Cu interacted with C60 in marine environment. This proposed project attempts to demonstrate that C60 can act as “Trojan horse” to transfer more environmental pollutants into cells. In turn, the co-exposure can induce addictive toxicity at different biological levels in marine mussels: Mytilus galloprovincialis. The result will shed light on the mechanism of the complex process in higher organisms, including humans.