本项目以艾灸“活血化瘀治未病”这一中医传统理论为出发点，以APOE-/-小鼠为模型，运用基因治疗载体腺相关病毒（AAV）感染血小板，特异性抑制或者激活血小板细胞，明确不同时间尺度下艾灸对血小板活化的特异性作用；应用新一代高通量测序技术（Next-Generation Sequencing (NGS)），观察艾灸后P2Y嘌呤能受体介导的血小板活化P2Y1-PLC和P2Y12-PI3K-Akt信号通路在mRNA和蛋白层次的表达，阐释艾灸防治动脉硬化的分子作用机制，筛选差异基因，丰富“中医治未病” 的科学内涵，满足我国中医药慢病防控的重大需求。

This project is based on the traditional Chinese traditional theory of moxibustion, "activating blood and removing blood stasis to prevent disease", and taking APOE-/- mouse as the model to define the effection of platelet activation infection or activating platelet In the method of gene therapy vector adeno-associated virus (AAV) specific infection or activating platelet. Application of a new Generation of high-throughput Sequencing technologies (Next Generation Sequencing (NGS), from P2Y purine and P2Y12 platelet activation P2Y1 receptor mediated - PLC - PI3K - Akt signaling pathways, and interpret molecular mechanism that moxibustion prevention and cure atherosclerosis, screening differential genes, and rich scientific connotation of "preventing disease", and to provide a scientific basis for clinical moxibustion prevention and cure atherosclerosis in clinic, meet the major needs of prevention cure of traditional Chinese medicine in our country.