土壤是抗生素抗性基因最重要的环境载体，通过有机肥进入土壤环境中的抗生素抗性基因会向植物迁移，生物质炭能够有效阻控其迁移，生物质炭阻控的规律、途径与机制是什么？针对这些科学问题，本项目在前期工作基础上，以抗生素抗性基因新型污染物为研究对象，联合高通量荧光定量PCR与测序技术，分别从室内模拟、温室盆栽和田间实验尺度，研究生物质炭阻控土壤中抗生素抗性基因向植物迁移的规律，探讨生物质炭加速土壤中抗生素抗性基因消解的动力学特征，探究生物质炭阻控抗生素抗性基因从土壤向植物迁移的途径，揭示生物质炭-根际微生物协同消解抗生素抗性基因的机制，探明田间条件下生物质炭阻控抗生素抗性基因从土壤向植物迁移的效率，阐明生物质炭阻控抗生素抗性基因在土壤-植物系统中迁移的原理。为控制抗生素抗性基因在土壤生态系统中的迁移转化提供理论依据与技术支撑，对保护生态环境与人类健康具有重要意义。

Soil is one of the most important environmental carriers of antibiotic resistance genes (ARGs). Manure application used to fertilize crops select for and increase transmission of ARGs from farm-to-fork. Previous studies have found that biochar amendment can effectively impede the transport of soil ARGs to plant. However, the kinetics, pathways, and mechanism of reducing ARGs via biochar amendment is still unknown. In the proposed project, hundreds of ARGs will be quantified using highly parallel quantitative PCR and characterized with a novel target enrichment strategy followed by second generation sequencing. Experiments will be performed at multiple levels including simulation in the laboratory, well-defined tests in the glasshouse, and field scale studies in the environment. Project objectives include: i) investigating the impedance control law of biochar amendment on the migration of ARGs from soil to plants, ii) explore the accelerated dissipation dynamics of ARGs in the soil with biochar amendment, iii) illustrate the migration pathway of ARGs from soil with and without biochar amendment to plant, iv) reveal the synergistic effect of biochar and rhizosphere microorganisms on the dissipation of soil ARGs, v) determine the impedance efficiency of biochar amendment on the migration of soil ARGs to the plants, vi) elucidate the impedance mechanisms of biochar amendment on the ARGs migration in soil-plant system. The results can provide theoretical basis and technical support for the control of ARGs in the soil ecosysterm, and will be of great significance on the preservation of ecological environment and human health.