PI3K/Akt/mTOR信号通路广泛参与肿瘤的发生发展，并成为肿瘤治疗的新靶点。研究表明，PI3K/Akt/mTOR信号通路的异常表达及磷酸化程度与胃癌的发生发展密切相关。我们前期研究证实,PI3K/Akt/mTOR信号通路上的mTOR基因多态性位点rs2295080可通过调控mTOR 基因表达的活性，影响胃癌发生的危险性。本课题拟通过生物信息学方法，运用两阶段分子流行病学病例-对照研究方法，探讨PI3K/Akt/mTOR信号通路关键基因上单核苷酸多态性（SNPs）与中国汉族人群胃癌易感的关联性。同时结合基因本体论等方法，应用生物信息学、分子克隆、报告基因、定点突变等技术,探讨各SNPs位点对所在基因表达的影响类型及其分子机制。在此基础上，采用eQTL方法系统的分析各SNPs位点的效应强度，为深入揭示胃癌发病机制提供理论依据，并为今后易感人群的筛查以及胃癌的个体化预防和干预提供重要参考。

PI3K/Akt/mTOR pathway is involving in the occurrence and development of cancers, which has been considered as a novel target in clinical therapy. Until now, accumulated studies have identified that aberrant expression and phosphorylation of PI3K/Akt/mTOR pathway were associated with risk of gastric cancer. Our previous results showed that rs2295080 polymorphisms of mTOR in the PI3K/Akt/mTOR pathway was associated with the risk of gastric cancer by regulating mTOR gene expression activity. In this study, we will use the bioinformatics to evaluate the associations between the function of SNPs in PI3K/Akt/mTOR pathway and the risk of gastric cancer in the Chinese population in two-stage case-control studies. Furthermore, the molecular biological methods including bioinformatics, molecular clones, reporter genes, site mutation and other methods, will be performed to confirm functional roles of these SNPs. Based on these results, we will systematically analyse these influences of the SNPs in gene expression and phosphorylation by eQTL methods. As a result, our data could reveal the mechanism of SNPs in gastric cancer and provide the important instruction for screening of susceptible individuals, personal prevention and intervention.